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Titolo:
Effect of temperature and calcium on transneuronal diffusion of DiI in fixed brain preparations
Autore:
Hofmann, MH; Bleckmann, H;
Indirizzi:
Univ Bonn, Inst Zool, D-53115 Bonn, Germany Univ Bonn Bonn Germany D-53115 iv Bonn, Inst Zool, D-53115 Bonn, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 88, anno: 1999,
pagine: 27 - 31
SICI:
0165-0270(19990401)88:1<27:EOTACO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONNECTIONS; NUCLEUS; HYPOTHALAMUS; GOLDFISH; LIGHT;
Keywords:
carbocyanine dye DiI; neuronal tracing technique; transneuronal transport; calcium; goldfish;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Hofmann, MH Univ Bonn, Inst Zool, Poppelsorfer Schloss, D-53115 Bonn, Germany Univ Bonn Poppelsorfer Schloss Bonn Germany D-53115 , Germany
Citazione:
M.H. Hofmann e H. Bleckmann, "Effect of temperature and calcium on transneuronal diffusion of DiI in fixed brain preparations", J NEUROSC M, 88(1), 1999, pp. 27-31

Abstract

The lipophilic tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was used to label neuronal pathways in fixed goldfish brains. The normal procedure involving 4% paraformaldehyde as a fixative, applying DiI and storing the brain in the fixative at 40 degrees C resulted in many cases in a rather diffuse labeling of fiber pathways and the occurrence of transneuronally labeled cells and fibers. We found that calcium andheat both facilitate the diffusion of DiI out of membranes in vibratome sections. We modified the protocol by adding the calcium binding substance ethylenediamine tetraacetate to all solutions and incubated at room temperature. This improved the sharpness of labeled structures and eliminated the transneuronal labeling in our material. Although transneuronal transport of the tracer may still occur under certain circumstances, the present modification of the DiI staining procedure substantially increased the staining quality and reproducibility and decreased the occurrence of transneuronal labeling. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:29:30