Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Induction of hyperphosphorylated tau in living slices of rat hippocampal formation and subsequent detection using an ELISA
Autore:
Mudher, AK; Woolley, ST; Perry, VH; Greene, JRT;
Indirizzi:
Univ Oxford, Dept Pharmacol, Oxford OX1 3QT, England Univ Oxford Oxford England OX1 3QT pt Pharmacol, Oxford OX1 3QT, England
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 88, anno: 1999,
pagine: 15 - 25
SICI:
0165-0270(19990401)88:1<15:IOHTIL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PAIRED HELICAL FILAMENTS; ELECTROPHYSIOLOGICALLY-DEFINED CLASSES; GLYCOGEN-SYNTHASE KINASE-3-BETA; CENTRAL NERVOUS-SYSTEM; HUMAN BRAIN TAU; ALZHEIMERS-DISEASE; TRANSGENIC MICE; IN-VITRO; PROTEIN PHOSPHATASES; OKADAIC ACID;
Keywords:
Alzheimer's disease; hyperphosphorylated tau; okadaic acid; hippocampus; electrophysiology; subiculum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Mudher, AK Inst Psychiat, De Crespigny Pk, Denmark Hill, London SE5 8AF, England Inst Psychiat De Crespigny Pk, Denmark Hill London England SE5 8AF
Citazione:
A.K. Mudher et al., "Induction of hyperphosphorylated tau in living slices of rat hippocampal formation and subsequent detection using an ELISA", J NEUROSC M, 88(1), 1999, pp. 15-25

Abstract

Although hyperphosphorylated tau is an established feature of Alzheimer's Disease, its role in the disease process is poorly understood, partly because of lack of suitable animal models. We describe the use of living slices of rat hippocampal formation to study tau phosphorylation. Using the AT8 antibody in an ELISA, phosphorylated tau was detected in freshly frozen slices and it increased significantly in slices that were incubated in an electrophysiological recording chamber; the amount detected was greatest when thehomogenisation buffer contained phosphatase and kinase inhibitors. The phosphorylated tau content of the slices increased significantly after exposure to the phosphatase 1 and 2A inhibitor okadaic acid (OA) - 1.5 mu M. Electrophysiological recordings confirmed that slices were alive and that OA hadno acute toxic effect. In control slices phosphorylated tau, detected immunohistochemically, was mainly in the somatodendritic compartment of neurones; in OA treated slices, there was an apparent decrease in somatodendritic AT8 staining and an increase in neuropil staining. Our system enables the induction of hyperhosphorylated tau within living slices, in an experimentalenvironment that can be used to study the biological consequences of such a change, and may therefore help further our understanding of the significance of hyperphosphorylated tau in Alzheimer's Disease. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 08:55:55