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Titolo:
Glutamate controls the induction of GABA-Mediated giant depolarizing potentials through AMPA receptors in neonatal rat hippocampal slices
Autore:
Bolea, S; Avignone, E; Berretta, N; Sanchez-Andres, JV; Cherubini, E;
Indirizzi:
Int Sch Adv Studies, Program Neurosci, I-34014 Trieste, Italy Int Sch Adv Studies Trieste Italy I-34014 urosci, I-34014 Trieste, Italy Int Sch Adv Studies, Ist Nazl Fis Mat Unit, I-34014 Trieste, Italy Int SchAdv Studies Trieste Italy I-34014 t Unit, I-34014 Trieste, Italy Univ Miguel Hernandez, Inst Bioingn, Dept Fisiol, Alicante 03550, Spain Univ Miguel Hernandez Alicante Spain 03550 Fisiol, Alicante 03550, Spain
Titolo Testata:
JOURNAL OF NEUROPHYSIOLOGY
fascicolo: 5, volume: 81, anno: 1999,
pagine: 2095 - 2102
SICI:
0022-3077(199905)81:5<2095:GCTIOG>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNAPTIC TRANSMISSION; CA2+-PERMEABLE AMPA; EXCITATORY ACTIONS; SPINAL NEURONS; CHANNELS; NMDA; MODULATION; CELLS; INTERNEURONS; NETWORK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Cherubini, E Intlych Adv Studies, Program Neurosci, Via Beirut 2-4, I-34014 Trieste, Ita Int Sch Adv Studies Via Beirut 2-4 Trieste Italy I-34014 Ita
Citazione:
S. Bolea et al., "Glutamate controls the induction of GABA-Mediated giant depolarizing potentials through AMPA receptors in neonatal rat hippocampal slices", J NEUROPHYS, 81(5), 1999, pp. 2095-2102

Abstract

Giant depolarizing potentials (GDPs) are generated by the interplay of thedepolarizing action of GABA and glutamate. In this study, single and dual whole cell recordings tin current-clamp configuration were performed from CA3 pyramidal cells in hippocampal slices obtained from postnatal (P) days P1- to pg-old rats to evaluate the role of ionotropic glutamate receptors inGDP generation. Superfusion of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)(10-40 mu M) completely blocked GDPs. However, in the presence of CNQX, itwas still possible to re-induce the appearance of GDPs with GABA (20 I-LM)or (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxadepropionate(AMPA) (5 mu M). This effect was prevented by the more potent and selective AMPA receptor antagonist GYKI 53655 (50-100 mu M). In the presence of GYKI 53655, both kainic or domoic acid (0.1-1 mu M) were unable to induce GDPs. In contrast, bath application of D-(-)-2-amino-5-phosphonopentanoicacid (50 mu M)or (+)-3-(2-carboxy-piperazin-4-yl)-propyl-L-phosphonic acid (20 mu M) Produced onlya 37 +/- 9% (SE) and 36 +/- 11% reduction in GDPs frequency, respectively. Cyclothiazide, a selective blocker of AMPA receptor desensitization, increased GDP frequency by 76 +/-: 14%. Experiments were also performed with an intracellular solution containing KF to block GABA, receptor-mediated responses. In these conditions, a,glutamatergic component of GDP was revealed. GDPs could still be recorded synchronous with those detected simultaneously with KCl-filled electrodes, although their amplitude was smaller. Similar results were found in pair recordings obtained from minislices containing only a small portion of the CA3 area. These data suggest that GDP generation requires activation of AMPA receptors by local release of glutamate from recurrent collaterals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:48:40