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Titolo:
Dopamine transporter-immunoreactive neurons decrease with age in the humansubstantia nigra
Autore:
Ma, SY; Ciliax, BJ; Stebbins, G; Jaffar, S; Joyce, JN; Cochran, EJ; Kordower, JH; Mash, DC; Levey, AI; Mufson, EJ;
Indirizzi:
RushRushsbyterian St Lukes Med Ctr, Dept Neurol Sci, Res Ctr Brain Repair,Rush Presbyterian St Lukes Med Ctr Chicago IL USA 60612 Ctr Brain Repair, Emory Univ, Dept Neurol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 Univ, Dept Neurol, Atlanta, GA 30322 USA ChristopherSAtr Parkinsons Dis Res, Sun Hlth Res Inst, Sun City, AZ 85378 U Christopher Ctr Parkinsons Dis Res Sun City AZ USA 85378 City, AZ 85378 U Univ Miami, Dept Neurol, Miami, FL 33101 USA Univ Miami Miami FL USA 33101 niv Miami, Dept Neurol, Miami, FL 33101 USA
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 409, anno: 1999,
pagine: 25 - 37
SICI:
0021-9967(19990621)409:1<25:DTNDWA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARKINSONISM-INDUCING NEUROTOXIN; ALZHEIMERS-DISEASE; MESSENGER-RNA; DISECTOR COUNTS; STEREOLOGICAL METHODS; MPTP NEUROTOXICITY; SINGLE SECTION; KNOCKOUT MICE; SPINAL-CORD; CELL LOSS;
Keywords:
aging; brainstem; dopamine; optical disector; immunocytochemistry; stereology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Mufson, EJ RushRushsbyterian St Lukes Med Ctr, Dept Neurol Sci, Res Ctr Brain Repair, Rush Presbyterian St Lukes Med Ctr 2242 W Harrison St Chicago IL USA 60612
Citazione:
S.Y. Ma et al., "Dopamine transporter-immunoreactive neurons decrease with age in the humansubstantia nigra", J COMP NEUR, 409(1), 1999, pp. 25-37

Abstract

Unbiased disector stereologic cell counting was applied to sections from the human substantia nigra that were immunostained by using a monoclonal antibody against the dopamine transporter (DAT). This antibody was found to penetrate the full thickness of the stained section. Quantification of the number of DAT immunostained neurons was performed in human cases stratified into three age groups, young (ages 0-49 years), middle aged (ages 50-69 years), and aged (ages 70-85 years). The number of DAT-immunoreactive nigral neurons was normalized for each case by constructing a ratio of the number ofDAT-containing neurons to total number of neuromelanin-containing cells ineach subject's sample. Three types of DAT nigral neurons were seen: type 1, intensely stained; type 2, lightly stained; and type 3, DAT-immunonegative neuromelanin-containing perikarya. By 50 years of age, the number of type1 neurons decreased significantly (P < 0.0001), whereas the number of type2 neurons increased with age (P < 0.0001). Type 3 neurons also increased with age (P < 0.01), although less robustly than type 2 neurons. Type 1 neurons decreased by 11.2% per decade, and the total number of nigral neurons (types 1-3) decreased by 6.7% per decade. Relative to the young group, therewere 75% and 88% reductions in type 1 neurons in the middle-aged and aged groups, respectively. This contrasts with the 35% and 41% reductions in total number of neuromelanin-containing neurons seen in middle-aged and aged groups, respectively. The young group had significantly more type I neurons and fewer type 2 neurons compared with middle-aged and aged participants. Past-hoc analyses indicated that the young group had significantly fewer type 3 neurons compared with middle-aged and aged participants. These findingsdemonstrate an age-related reduction in the number of substantia nigra DAT-immunoreactive neurons. Therefore, insight into the mechanisms regulating the rate of DAT synthesis may aid in our understanding of the decline of DATs with aging and its functional significance. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:03:16