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Titolo:
A controlled fed-batch cultivation for the production of new crude lipasesfrom Candida rugosa with improved properties in fine chemistry
Autore:
Sanchez, A; Ferrer, P; Serrano, A; Valero, F; Sola, C; Pernas, M; Rua, ML; Fernandez-Lafuente, R; Guisan, JM; de la Casa, R; Sinisterra, JV; Sanchez-Montero, JM;
Indirizzi:
Univ Autonoma Barcelona, Detp Engn Quim, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 E-08193 Barcelona, Spain Univ,Vigo, Fac Ciencias Ourense, Area Bioquim & Bioloxia Mol, Ourense 32004 Univ Vigo Ourense Spain 32004 Area Bioquim & Bioloxia Mol, Ourense 32004 Univainmplutense, Fac Farm, Dept Quim Organ & Farmaceut, E-28040 Madrid, Sp Univ Complutense Madrid Spain E-28040 an & Farmaceut, E-28040 Madrid, Sp CSIC, Inst Catalisis, Lab Tecnol Enzimat, E-28049 Madrid, Spain CSIC Madrid Spain E-28049 sis, Lab Tecnol Enzimat, E-28049 Madrid, Spain
Titolo Testata:
JOURNAL OF BIOTECHNOLOGY
fascicolo: 2-3, volume: 69, anno: 1999,
pagine: 169 - 182
SICI:
0168-1656(19990415)69:2-3<169:ACFCFT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
FATTY-ACIDS; CYLINDRACEA; PURIFICATION; HYDROLYSIS; ESTERS; GENES; ENHANCEMENT; ADSORPTION; ISOENZYMES; SEQUENCES;
Keywords:
lipase; Candida rugosa; production; characterisation; hydrolysis of HPBE; esterification of 2-aryl propionic derivatives; immobilisation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Valero, F Univ Autonoma Barcelona, Detp Engn Quim, E-08193 Barcelona, Spain Univ Autonoma Barcelona Barcelona Spain E-08193 rcelona, Spain
Citazione:
A. Sanchez et al., "A controlled fed-batch cultivation for the production of new crude lipasesfrom Candida rugosa with improved properties in fine chemistry", J BIOTECH, 69(2-3), 1999, pp. 169-182

Abstract

A controlled constant feeding rate fed-batch strategy using oleic acid as inducer produced a crude lipase preparation from Candida rugosa (CRL-UAB) with higher protein content, carbohydrate content and lipase activity than commercial Sigma type VII CRL. CRL-UAB was partially characterised and tested in selective biotransformations of chiral compounds in aqueous (2-hydroxy4-phenyl butanoic acid ethyl ester (HPBE)) and organic media (2-phenyl propionic acid and ketoprofen). CRL-UAB showed higher substrate specificity and enantioselectivity in aqueous media compared to Sigma CRL. Also, higher specific initial rates with 2-phenyl propionic acid and ketoprofen were observed in organic media. The influence of water on the esterification of ketoprofen was not relevant with CRL-UAB under the conditions tested, whereas adramatic influence was observed in Sigma CRL. Different CRL-UAB batches obtained under the same cultivation controlled conditions were identical fromthe point of view of chromatographic behaviour, immobilisation rates and catalytic properties, indicating that a reproducible C. rugosa lipase extract had been obtained. (C) 1999 Elsevier Science: B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 15:41:11