Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Staphylococcal toxic shock syndrome toxin-1 inhibits monocyte apoptosis
Autore:
Bratton, DL; May, KR; Kailey, JM; Doherty, DE; Leung, DYM;
Indirizzi:
Natl06ewish Med & Res Ctr, Dept Pediat, Div Allergy Immunol, Denver, CO 802 Natl Jewish Med & Res Ctr Denver CO USA 80206 rgy Immunol, Denver, CO 802 Natl Jewish Med & Res Ctr, Dept Med, Denver, CO 80206 USA Natl Jewish Med & Res Ctr Denver CO USA 80206 t Med, Denver, CO 80206 USA Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Ctr, Dept Pediat, Denver, CO 80262 USA Univ0536tucky, Med Ctr, Dept Med, Div Pulm & Crit Care Med, Lexington, KY 4 Univ Kentucky Lexington KY USA 40536 ulm & Crit Care Med, Lexington, KY 4
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 5, volume: 103, anno: 1999,
parte:, 1
pagine: 895 - 900
SICI:
0091-6749(199905)103:5<895:STSSTI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CELL-DEATH APOPTOSIS; ATOPIC-DERMATITIS; CYTOKINE PRODUCTION; CHEMOTACTIC FACTORS; T-CELLS; SKIN; IGE; PROLIFERATION; KERATINOCYTES;
Keywords:
monocyte; apoptosis; staphylococcal exotoxins; atopic dermatitis; granulocyte-macrophage colony-stimulating factor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Bratton, DL Natlt,Roomh Med & Res Ctr, Dept Pediat, Div Allergy Immunol, 1400 Jackson S Natl Jewish Med & Res Ctr 1400 Jackson St,Room D506 Denver COUSA 80206
Citazione:
D.L. Bratton et al., "Staphylococcal toxic shock syndrome toxin-1 inhibits monocyte apoptosis", J ALLERG CL, 103(5), 1999, pp. 895-900

Abstract

Background: Chronic atopic dermatitis (AD) lesions are associated with colonization by exotoxin-producing Staphylococcus aureus. Evidence suggests that cytokine production in AD, particularly of GM-CSF, prolongs survival of both peripheral blood monocytes and dermal monocyte-macrophages, the predominate inflammatory cell in lesions caused by chronic AD. Objective: We sought to determine whether the staphylococcal exotoxin, toxic shock syndrome toxin-l (TSST-1), could stimulate prosurvival cytokine production in monocytes and thereby inhibit apoptosis. Methods: Cultures of peripheral blood monocytes from normal donors and subjects with AD were incubated with various concentrations of TSST-1, and theincidence of apoptosis was assessed by examining cytospin preparations andthe appearance of hypodiploid DNA in the flow cytometer, Culture supernatants mere analyzed for GM-CSF, IL-1 beta, and TNF-alpha by ELISA,Results: TSST-1, in a concentration-dependent manner starting at 0.1 pg/mL, significantly inhibited monocyte apoptosis and resulted in the productionof the prosurvival cytokines GM-CSF, IL-1 beta, and TNF-alpha. In coculture conditions with conditioned media from TSST-1-stimulatcd monocytes, with or without neutralizing antibody to the various cytokines, the data show GM-CSF production was responsible for the inhibition of apoptosis,Conclusions: The data strongly suggest that staphylococcal exotoxins knownto colonize skin lesions on patients with chronic AD may induce the production of GM-CSF, resulting in inhibition of monocyte-macrophage apoptosis, and thereby contribute to the chronicity of this inflammatory disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 01:23:21