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Titolo:
Differentiation between Spitz nevi and malignant melanomas by interphase fluorescence in situ hybridization
Autore:
Wettengel, GV; Draeger, J; Kiesewetter, F; Schell, H; Neubauer, S; Gebhart, E;
Indirizzi:
Univ Erlangen Nurnberg, Inst Human Genet, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany Univ Erlangen Nurnberg, Dermatol Clin Policlin, D-91052 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91052 -91052 Erlangen, Germany
Titolo Testata:
INTERNATIONAL JOURNAL OF ONCOLOGY
fascicolo: 6, volume: 14, anno: 1999,
pagine: 1177 - 1183
SICI:
1019-6439(199906)14:6<1177:DBSNAM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUAMOUS-CELL CARCINOMA; PARAFFIN SECTIONS; FLOW-CYTOMETRY; DNA CONTENT; ABERRATIONS; CYTOGENETICS; FISH; HEAD; ABNORMALITIES; BENIGN;
Keywords:
I-FISH; Spitz nevi; malignant melanoma; numerical chromosomes aberrations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Gebhart, E Univgen,angen Nurnberg, Inst Human Genet, Schwabachanlage 10, D-91054 Erlan Univ Erlangen Nurnberg Schwabachanlage 10 Erlangen Germany D-91054
Citazione:
G.V. Wettengel et al., "Differentiation between Spitz nevi and malignant melanomas by interphase fluorescence in situ hybridization", INT J ONCOL, 14(6), 1999, pp. 1177-1183

Abstract

Spitz nevi are benign melanocytic neoplasias which have distinct pathological features that make the pathological differential diagnosis from malignant melanomas extremely difficult. The Spitz nevi may be misdiagnosed as malignant melanoma and vice versa. Therefore, interphase fluorescence irt situhybridization (I-FISH) was used for a possible discrimination between Spitz nevi and malignant melanomas on the basis of numerical aberrations of thechromosome complement in interphase nuclei of thin sections. Previous studies had shown changes in malignant melanomas which were not found at the same level in normal tissue or benign tumors. Thin sections of archival paraffin material from 42 Spitz nevi with different histological type and grade of anomaly were subjected to FISH-analyses using commercially available biotinylated and/or digoxigenated alphoid DNA probes of chromosomes 1, 6, 7, 9, 17 and 18, which were applied in combinations in a two- or three-color-FISH. Unaffected epithelial areas from the same sections served as 'controls'. The obtained data were compared with those collected previously from thinsections of malignant melanomas prepared in the same way. Due to the sometimes limited nevus area investigated, the number of evaluable nuclei was lower than expected from previous experiences with malignant melanomas. Therefore, only 20 nevi could be reliably evaluated. The comparison of the groupof Spitz nevi with the group of controls did not show any significant.

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Documento generato il 02/10/20 alle ore 01:42:30