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Titolo:
Environmental modulation of oral treponeme virulence in a murine model
Autore:
Kesavalu, L; Holt, SC; Ebersole, JL;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Periodont, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 Periodont, San Antonio, TX 78284 USA Univ Texas, Hlth Sci Ctr, Dept Microbiol, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 Microbiol, San Antonio, TX 78284 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 6, volume: 67, anno: 1999,
pagine: 2783 - 2789
SICI:
0019-9567(199906)67:6<2783:EMOOTV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORPHYROMONAS-GINGIVALIS W50; OUTER-MEMBRANE; IN-VIVO; BACTEROIDES-GINGIVALIS; BACTERIAL VIRULENCE; PATHOGENIC BACTERIA; ESCHERICHIA-COLI; DENTICOLA; HEMIN; IRON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Ebersole, JL UnivTXexas, Hlth Sci Ctr, Dept Periodont, 7703 Floyd Curl Dr,San Antonio, Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78284 tonio,
Citazione:
L. Kesavalu et al., "Environmental modulation of oral treponeme virulence in a murine model", INFEC IMMUN, 67(6), 1999, pp. 2783-2789

Abstract

This investigation examined the effects of environmental alteration on thevirulence of the oral treponemes Treponema denticola and Treponema pectinovorum. The environmental effects were assessed by using a model of localized inflammatory abscesses in mice. In vitro growth of T. denticola and T. pectinovorum as a function of modification of the cysteine concentration significantly enhanced abscess formation and size. In contrast, growth of T. denticola or T. pectinovorum under iron-limiting conditions (e.g., dipyridyl chelation) had no effect on abscess induction in comparison to that when the strains were grown under normal iron conditions. In vivo modulation of the microenvironment at the focus of infection with Cytodex beads demonstrated that increasing the local inflammation had no effect on lesion induction or size. In vivo studies involved the determination of the effects of increased systemic iron availability (e.g., iron dextran or phenylhydrazine) on the induction, kinetics, and size of lesions. T. denticola induced significantly larger lesions in mice with iron pretreatment and demonstrated systemic manifestations of the infectious challenge and an accompanying spreadinglesion with phenylhydrazine pretreatment (e.g., increases in circulating free hemoglobin). In contrast, T. pectinovorum virulence was minimally affected by this in vivo treatment to increase iron availability. T. denticola virulence, as evaluated by lesion size, was increased additively by in vivo iron availability, and cysteine modified growth of the microorganism. Additionally, galactosamine sensitized mice to a lethal outcome following infection with both T. denticola and T. pectinovorum, suggesting an endotoxin-like activity in these treponemes. These findings demonstrated the ability to modify the virulence capacity of T. denticola and T. pectinovorum by environmental conditions which can be evaluated by using in vivo murine models.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:36:30