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Titolo:
N-terminal domain, residues 1-91, of ribosomal protein TL5 from Thermus thermophilus binds specifically and strongly to the region of 5S rRNA containing loop E
Autore:
Gongadze, GM; Meshcheryakov, VA; Serganov, AA; Fomenkova, NP; Mudrik, ES; Jonsson, BH; Liljas, A; Nikonov, SV; Garber, MB;
Indirizzi:
Russian Acad Sci, Inst Prot Res, Pushchino 142292, Moscow Region, Russia Russian Acad Sci Pushchino Moscow Region Russia 142292 cow Region, Russia Umea Univ, Dept Biochem, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 a Univ, Dept Biochem, S-90187 Umea, Sweden Univ Lund, Dept Mol Biophys, S-22100 Lund, Sweden Univ Lund Lund Sweden S-22100 nd, Dept Mol Biophys, S-22100 Lund, Sweden
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 451, anno: 1999,
pagine: 51 - 55
SICI:
0014-5793(19990514)451:1<51:NDR1OR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBONUCLEASE-RESISTANT FRAGMENT; ESCHERICHIA-COLI RIBOSOMES; CRYSTAL-STRUCTURE; BACILLUS-STEAROTHERMOPHILUS; 50S SUBUNIT; 5-S RNA; COMPLEXES; L25; CRYSTALLIZATION; IDENTIFICATION;
Keywords:
ribosomal protein TL5; 5S rRNA-protein complex; loop E-helix IV domain of 5SrRNA; crystallization; Thermus thermophilus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Gongadze, GM Russian Acad Sci, Inst Prot Res, Pushchino 142292, Moscow Region, Russia Russian Acad Sci Pushchino Moscow Region Russia 142292 ussia
Citazione:
G.M. Gongadze et al., "N-terminal domain, residues 1-91, of ribosomal protein TL5 from Thermus thermophilus binds specifically and strongly to the region of 5S rRNA containing loop E", FEBS LETTER, 451(1), 1999, pp. 51-55

Abstract

In this work we show for the first time that the overproduced N-terminal fragment (residues 1-91) of ribosomal protein TL5 binds specifically to 5S rRNA and that the region of this fragment containing residues 80-91 is a necessity for its RNA-binding activity. The fragment of Escherichia coli 5S rRNA protected by TL5 against RNase A hydrolysis was isolated and sequenced. This 39 nucleotides fragment contains loop E and helices IV and V of 5S rRNA, The isolated RNA fragment forms stable complexes with TL5 and its N-terminal domain. Crystals of TL5 in complex with the RNA fragment diffracting to 2.75 Angstrom resolution were obtained. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:13:23