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Titolo:
IN-VITRO ANALYSIS OF OOCYTE CUMULUS COMPLEX PICKUP RATE IN THE HAMSTER MESOCRICETUS-AURATUS
Autore:
HUANG S; DRIESSEN N; KNOLL M; TALBOT P;
Indirizzi:
UNIV CALIF RIVERSIDE,DEPT BIOL RIVERSIDE CA 92521 UNIV CALIF RIVERSIDE,DEPT BIOL RIVERSIDE CA 92521
Titolo Testata:
Molecular reproduction and development
fascicolo: 3, volume: 47, anno: 1997,
pagine: 312 - 322
SICI:
1040-452X(1997)47:3<312:IAOOCC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOMETRIOSIS PERITONEAL-FLUID; CIGARETTE-SMOKE; RABBIT OVIDUCT; OVUM TRANSPORT; CILIA; CAPTURE; MUSCLE;
Keywords:
OOCYTE CUMULUS COMPLEX; OVIDUCT; GAMETE TRANSPORT; CILIA; HAMSTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
S. Huang et al., "IN-VITRO ANALYSIS OF OOCYTE CUMULUS COMPLEX PICKUP RATE IN THE HAMSTER MESOCRICETUS-AURATUS", Molecular reproduction and development, 47(3), 1997, pp. 312-322

Abstract

In mammals, the oocyte and its surrounding cumulus cells constitute an oocyte cumulus complex (OCC). During ovulation, OCCs are extruded into the peritoneal or bursal cavity, depending on the species, and are then rapidly picked up by the fimbria on the outer surface of the oviductal infundibulum and transported to the ampulla, where fertilizationoccurs. We developed a method to measure OCC pickup rates quantitatively in vitro, and we used this method to evaluate the effects of viscosity and temperature on pickup rates. Hamster infundibula are placed in a holding pipette in a chamber modified to study OCC pickup. Ciliarybeat frequencies (CBF) can be measured in the same preparation. Pickup rates vary depending on the pathway on which the OCC travels over the surface of the infundibulum; however, rates for a given pathway are very consistent. The average pickup rate at room temperature calculated from three different pathways/infundibulum was 55.2 +/- 10.6 mu m/sec. Both rates between infundibula from the same female and rates amonginfundibula from different females were in most cases similar. Preparations preincubated in vitro for 2.75 hr produced rates similar to nonpreincubated samples, while longer preincubation resulted in decreasedrates. Inclusion of Ficoll in culture medium to increase viscosity caused a concentration-dependent decrease in both OCC pickup rate and inCBF. However, a significant decrease in OCC pickup rate was only observed at viscosities higher than those found in bursal fluid. When trials were run at physiological temperature (36.4 degrees C) rather than ambient temperature, rates increased to 136.7 +/- 29.9 (SD) mu m/sec. Linear regression analysis demonstrated a strong positive correlation (r = 0.94) between OCC pickup rate and temperature. The OCC pickup rate assay can be used experimentally, and should be valuable in evaluating factors that affect rate and in studies dealing with the mechanism of OCC pickup. (C) 1997 Wiley-Liss, Inc.

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Documento generato il 04/12/20 alle ore 22:11:59