Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Enhancement of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin fluorescence emission by inclusion in natural and modified cyclodextrins
Autore:
Demore, D; Kasselouri, A; Bourdon, O; Blais, J; Mahuzier, G; Prognon, P;
Indirizzi:
Univlabry, Sud, Ctr Etud Pharmaceut, Lab Chim Analyt 2, F-92296 Chatenay Ma Univ Paris Sud Chatenay Malabry France F-92296 yt 2, F-92296 Chatenay Ma UnivsParis 06, Lab Physicochim Biomol & Cellulaire, CNRS, ESA, F-75252 Pari Univ Paris 06 Paris France 05 omol & Cellulaire, CNRS, ESA, F-75252 Pari
Titolo Testata:
APPLIED SPECTROSCOPY
fascicolo: 5, volume: 53, anno: 1999,
pagine: 523 - 527
SICI:
0003-7028(199905)53:5<523:EO5FE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTODYNAMIC THERAPY; LIQUID-CHROMATOGRAPHY; AFLATOXINS; TISSUES; CANCER;
Keywords:
cyclodextrins; 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC); fluorescence; inclusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kasselouri, A Univlabry, Sud, Ctr Etud Pharmaceut, Lab Chim Analyt 2, F-92296 Chatenay Ma Univ Paris Sud Chatenay Malabry France F-92296 Chatenay Ma
Citazione:
D. Demore et al., "Enhancement of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin fluorescence emission by inclusion in natural and modified cyclodextrins", APPL SPECTR, 53(5), 1999, pp. 523-527

Abstract

Fluorescence properties of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (m-THPC), a sensitizer used in photodynamic therapy (PDT), were studied in the presence of three native cyclodextrins (CDs)-alpha-, beta-, and gamma-CD-and two modified cyelodextrins-heptakis (2,6-di-O-methyl) beta-CD (Me-beta-CD) and heptakis (2-O-hydroxy-propyl) beta-CD (HP-beta-CD), The CDs studied have been shown to undergo the formation of an inclusion complex with m-THPC, leading to a large enhancement (up to 300 times) of m-THPC fluorescence intensity depending on the CD used. Stoichiometry and association constants have been determined on the basis of the variation of fluorescence intensity. A 1:2 stoichiometry has been found for m-THPC complexes with gamma-CD, Me-beta-CD, and HP-beta-CD, Association constants as high as K = 9.5 x 10(8) M-2 in the case of mTHPC/2Me-beta-CD and K = 2.7 x 10(9) M-2 in the case of m-THPC/2HP-beta-CD complex were determined, whereas a lower value (K = 2.4 x 10(4) M (2)) was found in the case of m-THPC/2 gamma-CD, Because of the highest fluorescence enhancement (a factor of about 300) observed with Me-beta-CD, this compound would be the most suitable to improve the detection ofm-THPC in further pharmacokinetics and biodistribution studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 11:47:03