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Titolo:
The feasibility of using blood bank-stored (4 degrees C) cord blood, unmatched for HLA for marrow transplantation
Autore:
Ende, N; Lu, S; Mack, R; Ponzio, NM;
Indirizzi:
Univ3Med & Dent New Jersey, Sch Med, Dept Pathol & Lab Med, Newark, NJ 0710 Univ Med & Dent New Jersey Newark NJ USA 07103 & Lab Med, Newark, NJ 0710 ShanghaiaMed Univ, Hua Shan Hosp, Dept Med, Shanghai 200032, Peoples R Chin Shanghai Med Univ Shanghai Peoples R China 200032 200032, Peoples R Chin
Titolo Testata:
AJCP. American journal of clinical pathology
fascicolo: 6, volume: 111, anno: 1999,
pagine: 773 - 781
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGENITOR CELLS; BONE-MARROW; HEMATOPOIETIC PROGENITORS; IRRADIATION; CULTURES;
Keywords:
human cord blood; blood bank-stored; marrow transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ende, N Unive,ed & Dent New Jersey, Sch Med, Dept Pathol & Lab Med, 185 S Orange Av Univ Med & Dent New Jersey 185 S Orange Ave Newark NJ USA 07103 Av
Citazione:
N. Ende et al., "The feasibility of using blood bank-stored (4 degrees C) cord blood, unmatched for HLA for marrow transplantation", AM J CLIN P, 111(6), 1999, pp. 773-781

Abstract

The main purpose for storing large numbers of umbilical cord blood (CB) units by cryopreservation is to obtain a close HLA match for use in bone marrow transplantation. The use of partially matched or unmatched CB has been suggested, and publications about the success of 3 antigen mismatches have given some credence to this suggestion. Graft vs host disease still is considered a major barrier for successful CB transplantation. The cost per frozen CB unit of approximately $15,000 considerably Limits its availability in developing countries. Eleven human umbilical cord specimens were stored in gas-permeable bags at 4 degrees C for up to 3 weeks. Clonal growth, replating efficiency in methylcellulose cultures, differential count, and flow cytometric immunophenotyping results were examined at intervals up to 21 days. Mixed lymphocyte cultures were evaluated on 13 similarly stored specimens at intervals tip to 14 days. When plated at 1, 10 and 21 days, the combinedpercentage of the more primitive colonies increased on days 10 and 21. Replating efficiency of blast cell colonies when stem cell factor was added was 81.2% and 67.8% on days 10 and 21, respectively. When mononuclear cells were immunophenotyped, the mean percentage of CD34(+) and CD117(+) cells, considered primitive stem cell markers, increased significantly from day 1 today 21. The ability of stored CB cells to respond to phytohemagglutinin oralloantigens decreased progressively from day 1 to day 14 By day 14 the reactivity of CB responder cells, in mixed lymphocyte cultures, to fresh allogeneic CB stimulator cells declined significantly. These findings suggest that CB can be stored in existing blood bank facilities and retain ifs hematopoietic potential for transplantation. Furthermore, it may be feasible to combine individual CB samples to provide a sufficient number of viable stemcells for transplantation, substantially expanding the number of potentialrecipients.

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Documento generato il 05/07/20 alle ore 10:24:28