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Titolo:
The cellular kinase binding motifs (PxxP and RR) in human immunodeficiencyvirus type 1 Nef protein are dispensable for producer-cell-dependent enhancement of viral entry
Autore:
Tokunaga, K; Ikuta, K; Adachi, A; Matsuda, M; Kurata, T; Kojima, A;
Indirizzi:
Natl Inst Infect Dis, Dept Pathol, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 pt Pathol, Tokyo 1628640, Japan Hokkaidoanniv, Inst Immunol Sci, Sect Serol, Sapporo, Hokkaido 0600815, Jap Hokkaido Univ Sapporo Hokkaido Japan 0600815 pporo, Hokkaido 0600815, Jap Univ Tokushima, Sch Med, Dept Virol, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 t Virol, Tokushima 7708503, Japan Int Med Ctr Japan, Dept Pathol, Res Inst, Shinjuku Ku, Tokyo 1628655, Japan Int Med Ctr Japan Tokyo Japan 1628655 Shinjuku Ku, Tokyo 1628655, Japan
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 257, anno: 1999,
pagine: 285 - 289
SICI:
0042-6822(19990510)257:2<285:TCKBM(>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROVIRAL DNA-SYNTHESIS; HIV-1 NEF; RHESUS MACAQUES; DOWN-REGULATION; IN-VIVO; MUTATIONAL ANALYSIS; GENE; REPLICATION; AIDS; CD4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kojima, A Natl Inst Infect Dis, Dept Pathol, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 Tokyo 1628640, Japan
Citazione:
K. Tokunaga et al., "The cellular kinase binding motifs (PxxP and RR) in human immunodeficiencyvirus type 1 Nef protein are dispensable for producer-cell-dependent enhancement of viral entry", VIROLOGY, 257(2), 1999, pp. 285-289

Abstract

We have recently demonstrated that human immunodeficiency virus type 1 (HIV-I) Nef is required for enhancing viral infectivity by increasing the efficiency of viral entry in a producer-cell-dependent manner, suggesting the possible involvement of a cellular factor(s) in the enhancement of Viral entry. Moreover, it has been reported that a proline-rich (PxxP) motif and an Arg-Arg (RR) motif in HIV-1 Nef bind to the SH3 domain of the Src-family tyrosine kinase Hck and to a serine/threonine kinase, respectively. To address whether these cellular kinase binding motifs, PxxP and RR, could be involved in Virus producer-cell-dependent enhancement of viral entry, we constructed two nef mutant proviral clones in which these motifs were mutated. Theresults show that the HIV-I Nef PxxP motif, which significantly influencedviral infectivity, and the RR motif, which modestly affected viral infectivity, were both dispensable for enhanced viral entry, thus suggesting that another interaction of Nef with a cellular factor(s) is involved in the efficiency of viral entry. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 13:18:46