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Titolo:
CHARACTERIZATION OF HIV-RELATED PERIODONTITIS IN AIDS PATIENTS - HIV-INFECTED MACROPHAGE EXUDATE IN GINGIVAL CREVICULAR FLUID AS A HALLMARKOF DISTINCTIVE ETIOLOGY
Autore:
SUZUKI T; TAI H; YOSHIE H; JEANNEL D; FOURNIER S; DUPONT B; DETHE G; HARA K;
Indirizzi:
NIIGATA UNIV,SCH DENT,DEPT PERIODONTOL,2-5274 GAKKO CHO NIIGATA 951 JAPAN NIIGATA UNIV,SCH DENT,DEPT PERIODONTOL NIIGATA 951 JAPAN INST PASTEUR,EPIDEMIOL ONCOGEN VIRUS UNIT PARIS FRANCE PASTEUR INST HOSP PARIS FRANCE
Titolo Testata:
Clinical and experimental immunology
fascicolo: 2, volume: 108, anno: 1997,
pagine: 254 - 259
SICI:
0009-9104(1997)108:2<254:COHPIA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ADULT PERIODONTITIS; T-CELLS; REPLICATION; EXPRESSION; MONOCYTES; DISEASES; SUBSETS;
Keywords:
MACROPHAGES; AIDS-RELATED PATHOLOGY; INFLAMMATORY TISSUE EXUDATE; PERIODONTITIS; VIRAL REPLICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
T. Suzuki et al., "CHARACTERIZATION OF HIV-RELATED PERIODONTITIS IN AIDS PATIENTS - HIV-INFECTED MACROPHAGE EXUDATE IN GINGIVAL CREVICULAR FLUID AS A HALLMARKOF DISTINCTIVE ETIOLOGY", Clinical and experimental immunology, 108(2), 1997, pp. 254-259

Abstract

In an attempt to clarify the immunobiological events featuring periodontitis lesions of AIDS patients in the late stage of the disease, peripheral blood (PB) and gingival crevicular fluid (GCF) leucocytes fromperiodontitis lesions of 23 late-stage AIDS patients were analysed bythree-colour flow cytometry for detection and identification of intracytoplasmic p24(+) cell fractions. The cells were reacted with CD14 and CD68 for mononuclear phagocytes or with CD4 and CD14 for Th cells, then with anti-p24 MoAb. To detect HIV proviral sequences and intracellular p24 RNA sequences, genomic DNA and cellular RNA from leucocytes were extracted for semi-nested polymerase chain reaction (PCR) amplification. CD68(+)/p24(+) and CD14(+)/CD68(+)/p24(+) fractions were largerin GCF than in PB (P < 0.0001; P < 0.003). CD14(+)/p24(+) fraction was lower in GCF than in PB (P < 0.05). The fluorescence intensities (FI) for intracellular p24 in CD68(+) and CD14(+)/CD68(+) cells were higher in GCF than in PB (P < 0.003; P < 0.02), whereas those of CD14(+) macrophages did not differ. The p24 FI of CD68(+) macrophages in GCF correlated with CD4(+) lymphocyte counts in PB (P < 0.005). p24 FI levels of CD14(+) monocytes in GCF and PB significantly correlated (P < 0.02), whereas that of CD68(+) macrophages did not. PCR and reverse transcriptase (RT)-PCR of cellular DNA and RNA yielded positive signals, demonstrating viral integration and production in GCF leucocytes. These results show that periodontitis lesions in AIDS patients can be characterized by a rapid macrophage turnover, and these HIV-infected macrophage exudates in GCF may be considered as a within-mouth source of virus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:11:42