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Titolo:
Possible involvement of cAMP in aerial mycelium formation and secondary metabolism in Streptomyces griseus
Autore:
Kang, DK; Li, XM; Ochi, K; Horinouchi, S;
Indirizzi:
Univ657,yo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138 Univ Tokyo Tokyo Japan 1138657 i, Dept Biotechnol, Bunkyo Ku, Tokyo 1138 Natl Food Res Inst, Tsukuba, Ibaraki 30586, Japan Natl Food Res Inst Tsukuba Ibaraki Japan 30586 kuba, Ibaraki 30586, Japan
Titolo Testata:
MICROBIOLOGY-UK
, volume: 145, anno: 1999,
parte:, 5
pagine: 1161 - 1172
SICI:
1350-0872(199905)145:<1161:PIOCIA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR RECEPTOR PROTEIN; FACTOR-BINDING PROTEIN; A-FACTOR BIOSYNTHESIS; ADENYLATE-CYCLASE; COELICOLOR A3(2); CYCLIC-AMP; PHOSPHOTRANSFERASE GENE; AUTOREGULATORY FACTORS; STRINGENT RESPONSE; ESCHERICHIA-COLI;
Keywords:
A-factor; A-factor receptor protein; protein tyrosine phosphorylation; stringent response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Horinouchi, S Univ657,yo, Grad Sch Agr & Life Sci, Dept Biotechnol, BunkyoKu, Tokyo 1138 Univ Tokyo Tokyo Japan 1138657 hnol, Bunkyo Ku, Tokyo 1138
Citazione:
D.K. Kang et al., "Possible involvement of cAMP in aerial mycelium formation and secondary metabolism in Streptomyces griseus", MICROBIO-UK, 145, 1999, pp. 1161-1172

Abstract

In Streptomyces griseus, A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) triggers secondary metabolism and morphogenesis by binding a repressor protein (ArpA) and dissociating it from DNA, UV-mutagenesis of the A-factor-deficient mutant HH1 generated strain HO2, defective in the synthesis of ArpA and therefore able to form aerial mycelium, spores and streptomycin. Shotgun cloning of chromosomal DNA from wild-type S, griseus in strain HO2 yielded a gene that suppressed aerial mycelium formation and streptomycin production. Nucleotide sequencing and subcloning revealed that the gene encoded a eukaryotic-type adenylate cyclase (CyaA), In mutant HO2 production of cAMP was growth-dependent until the middle of the exponential growth stage; the production profile was the same as in the wildtype strain. However, the amount of cAMP produced was five times larger when mutant HO2 harboured cyaA on the high-copy-number plasmid pIJ486. Consistent with this, supplying cAMP exogenously at a high concentration to mutant HO2 suppressedformation of both aerial mycelium and streptomycin. On the other hand, some lower concentrations of cAMP stimulated or accelerated aerial mycelium formation. No effects of exogenous cAMP on morphogenesis and secondary metabolism were apparent in the wild-type strain. In addition, disruption of the chromosomal cyaA gene in the wild-type strain had almost no effect. Introducing cyaA cloned in either a low- or a high-copy-number plasmid suppressed morphogenesis and secondary metabolism not only in mutant HO2 but also in other arpA mutants, implying that the effects of cAMP became apparent in thearpA-defective background. When mutant HO2 carried cyaA on a plasmid, synthesis of the stringent response factor ppGpp was greatly reduced; this may account for the observed suppression by cAMP of morphogenesis and secondarymetabolism, cAMP also affected protein tyrosine phosphorylation, as determined with anti-phosphotyrosine antibody.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 15:15:32