Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Dopamine D1-like receptor subtypes in human peripheral blood lymphocytes
Autore:
Ricci, A; Bronzetti, E; Mignini, F; Tayebati, SK; Zaccheo, D; Amenta, F;
Indirizzi:
Univ,Camerino, Dipartimento Sci Farmacol & Med Sperimentale, Sez Anat Umana Univ Camerino Camerino Italy I-62032 & Med Sperimentale, Sez Anat Umana Univalyme La Sapienza, Dipartimento Sci Cardiovasc & Resp, I-00185 Rome, It Univ Rome La Sapienza Rome Italy I-00185 iovasc & Resp, I-00185 Rome, It Univ Genoa, Dipartimento Med Sperimentale, Sez Anat Umana, Genoa, Italy Univ Genoa Genoa Italy o Med Sperimentale, Sez Anat Umana, Genoa, Italy
Titolo Testata:
JOURNAL OF NEUROIMMUNOLOGY
fascicolo: 2, volume: 96, anno: 1999,
pagine: 234 - 240
SICI:
0165-5728(19990503)96:2<234:DDRSIH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIOLIGAND BINDING; MOLECULAR-BIOLOGY; LIMBIC SYSTEM; RAT-BRAIN; IDENTIFICATION; CATECHOLAMINES; BROMOCRIPTINE; EXPRESSION; STRESS; SITES;
Keywords:
peripheral blood lymphocytes; dopamine receptors; D5 receptor; D1 receptor; in situ hybridization cytochemistry; anti-receptor antibodies; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Amenta, F Univ,Camerino, Dipartimento Sci Farmacol & Med Sperimentale, SezAnat Umana Univ Camerino Via Scalzino 3 Camerino Italy I-62032 Anat Umana
Citazione:
A. Ricci et al., "Dopamine D1-like receptor subtypes in human peripheral blood lymphocytes", J NEUROIMM, 96(2), 1999, pp. 234-240

Abstract

Molecular biology studies have shown that human peripheral blood lymphocytes express a dopamine D5 receptor, whereas no information is available on dopamine DI receptor, the other dopamine D1-like receptor subtype. Radioligand binding assay investigations with the nonsubtype selective dopamine D1-like receptor antagonist [H-3]SCH 23390 as radioligand have suggested the presence of a dopamine D5 receptor in human peripheral blood lymphocytes. However. so far no evidence was provided as whether or not human peripheral blood lymphocytes express a dopamine D1 receptor. In this study, we have investigated dopamine D1 and D5 receptor mRNA and the influence of antibodies against dopamine D1 and D5 receptors on [H-3]SCH 23390 binding to intact human peripheral blood lymphocytes. The two receptors were also analyzed by immunocytochemistry. Dopamine D5 receptor, but not D1 mRNA, was detected in human peripheral blood lymphocytes. Anti-dopamine D5 receptor antibodies, but not anti-dopamine D1 receptor antibodies, significantly decreased [H-3]SCH 23390 binding to human peripheral blood lymphocytes. A dark-brown immunoreactivity was visualized in cytospin centrifuged human peripheral blood lymphocytes exposed to anti-dopamine D5, but not to anti-dopamine D1 receptor antibodies. These data collectively indicate that dopamine D5 receptor is the only dopamine D1-like receptor subtype expressed by human peripheral blood lymphocytes. (C) 1999 Elsevier Science. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 07:57:21