Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Translocation and down-regulation of protein kinase C-alpha, -beta, and -gamma isoforms during ischemia-reperfusion in rat brain
Autore:
Harada, K; Maekawa, T; Abu Shama, KM; Yamashima, T; Yoshida, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Legal Med, Yamaguchi 7558505, Japan Yamaguchi Univ Yamaguchi Japan 7558505 gal Med, Yamaguchi 7558505, Japan Yamaguchi Univ, Sch Med, Dept Emergency & Crit Care Med, Yamaguchi 7558505, Yamaguchi Univ Yamaguchi Japan 7558505 Crit Care Med, Yamaguchi 7558505, Kanazawa Univ, Sch Med, Dept Neurosurg, Kanazawa, Ishikawa 920, Japan Kanazawa Univ Kanazawa Ishikawa Japan 920 , Kanazawa, Ishikawa 920, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 6, volume: 72, anno: 1999,
pagine: 2556 - 2564
SICI:
0022-3042(199906)72:6<2556:TADOPK>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; PHORBOL-ESTER; POSTISCHEMIC REPERFUSION; NEUROTRANSMITTER RELEASE; NUCLEAR TRANSLOCATION; FOREBRAIN ISCHEMIA; CEREBRAL-ISCHEMIA; CALPAIN; PROTEOLYSIS; INVOLVEMENT;
Keywords:
protein kinase C isoform; translocation; down-regulation; dephosphorylation; calpain; ischemia-reperfusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshida, K Yamaguchiapanv, Sch Med, Dept Legal Med, 1144 Kogushi, Yamaguchi 7558505, J Yamaguchi Univ 1144 Kogushi Yamaguchi Japan 7558505 7558505, J
Citazione:
K. Harada et al., "Translocation and down-regulation of protein kinase C-alpha, -beta, and -gamma isoforms during ischemia-reperfusion in rat brain", J NEUROCHEM, 72(6), 1999, pp. 2556-2564

Abstract

We investigated the distribution of protein kinase C (PKC) isoforms in thesubcellular fractions (P1, 1,000-g pellet; P2, 10,000-g pellet; P3, 100,000-g pellet; S, 100,000-g supernatant) of rat forebrain after ischemia or reperfusion by immunoblotting. PKC-delta and -epsilon isoforms were predominant in the P2 (synaptosome-rich) fraction, whereas PKC-alpha, -beta, -gamma,-epsilon, and -zeta isoforms were rich in the S (cytosolic) fraction. Withtime of ischemia (5-30 min), PKC-alpha, -beta, and -gamma translocated to the P2 and P3 fractions, whereas reperfusion for 60 min after 30 min of ischemia reduced PKC-beta activity greatly and PKC-alpha and -gamma activitiesto a lesser extent. There was no redistribution of PKC-delta, -epsilon, and -zeta after ischemia or reperfusion. A calpain inhibitor, acetylleucylleucylnorleucinal, inhibited the down-regulation of PKC-beta, through intravenous injection. The PKC translocation to the P2 fraction was accompanied by their dephosphorylation, transition of PKC-alpha from dimer to trimer, and the decrease in activity. These data show that PKC-alpha, -beta, and -gammaisoforms translocate chiefly to the synaptosome in ischemic brain in association with the dephosphorylation, multimeric change, and inactivation, followed by the proteolysis of PKC-beta by calpain after postischemic reperfusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:58:37