Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Nerve growth factor determines survival and death of PC12 cells by regulation of the bcl-x, bax, and caspase-3 genes
Autore:
Rong, P; Bennie, AM; Epa, WR; Barrett, GL;
Indirizzi:
Univ Melbourne, Dept Physiol, Parkville, Vic 3052, Australia Univ Melbourne Parkville Vic Australia 3052 arkville, Vic 3052, Australia Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia Walter & Eliza Hall Inst Med Res Melbourne Vic Australia 3050 , Australia
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 6, volume: 72, anno: 1999,
pagine: 2294 - 2300
SICI:
0022-3042(199906)72:6<2294:NGFDSA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROTROPHIN RECEPTOR; NEURONAL DEATH; DEFICIENT MICE; APOPTOSIS; FAMILY; P75; ACTIVATION; CED-4; DIFFERENTIATION; SPECIFICITIES;
Keywords:
apoptosis; nerve growth factor; caspase; bcl-xl; bax; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Barrett, GL Univ Melbourne, Dept Physiol, Parkville, Vic 3052, Australia Univ Melbourne Parkville Vic Australia 3052 c 3052, Australia
Citazione:
P. Rong et al., "Nerve growth factor determines survival and death of PC12 cells by regulation of the bcl-x, bax, and caspase-3 genes", J NEUROCHEM, 72(6), 1999, pp. 2294-2300

Abstract

We investigated the effects of nerve growth factor (NGF) and NGF withdrawal on expression of members of the bcl-2 family of genes and caspase-3 in PC12 cells. NGF regulated several members of the bcl-2 family and caspase-3 in a manner consistent with its effect on apoptosis in PC12 cells. Levels ofbcl-xl, bcl-xs, and caspase-3 mRNAs were increased by NGF treatment. The increases in caspase-3 and bcl-xs levels should have disposed the cells toward apoptosis but were opposed by the simultaneous increase in bcl-xl level. NGF withdrawal resulted in abrupt down-regulation of bcl-xl and up-regulation of bax, favoring apoptosis. Forced expression of bcl-xl after NGF withdrawal was sufficient to prevent cell death. Cell death was rapid when NGF was withdrawn after 5 days of treatment but relatively slow when NGF was withdrawn after only 1 or 2 days of treatment. This was consistent with the reduced accumulation of caspase-3 mRNA with shorter NGF treatments. These results indicate that Bcl-xl, Bcl-xs, Bar, and caspase-3 are important regulators of apoptosis in PC12 cells. Furthermore, regulation of their mRNA levels is implicated in the signal transduction of NGF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:47:35