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Titolo:
Use of base excision sequence scanning for detection of genetic variationsin St. Louis encephalitis virus isolates
Autore:
Charrel, RN; Levy, N; Tesh, RB; Chandler, LJ;
Indirizzi:
INSERM, Fac Med, U491, Marseille, France INSERM Marseille FranceINSERM, Fac Med, U491, Marseille, France Univ Texas, Med Branch, Dept Pathol, Ctr Trop Dis, Galveston, TX 77555 USAUniv Texas Galveston TX USA 77555 , Ctr Trop Dis, Galveston, TX 77555 USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 6, volume: 37, anno: 1999,
pagine: 1935 - 1940
SICI:
0095-1137(199906)37:6<1935:UOBESS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-C VIRUS; STRAND CONFORMATION POLYMORPHISM; HETERODUPLEX MOBILITY ASSAY; CHAIN-REACTION PRODUCTS; STRAINS; GENOTYPES; RESPONSES; INFECTION; VARIANTS; HIV-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Charrel, RN Univeston,, Med Branch, Dept Pathol, Ctr Trop Dis, Keiller Bldg 3-150, Galv Univ Texas Keiller Bldg 3-150 Galveston TX USA 77555 150, Galv
Citazione:
R.N. Charrel et al., "Use of base excision sequence scanning for detection of genetic variationsin St. Louis encephalitis virus isolates", J CLIN MICR, 37(6), 1999, pp. 1935-1940

Abstract

Twenty-two isolates of St. Louis encephalitis (SLE) virus of various geographical origins (Brazil, Argentina, Panama, Texas, Missouri, Maryland, California, and Florida) were examined for genetic variation by the base excision sequence scanning (BESS T-scan) method. A fragment was amplified in the envelope gene with the forward primer labeled in the PCR The BESS T-scan method determined different clusters according to the profiles generated for the isolates and successfully grouped the isolates according to their geographical origins. Two major clusters, the North American cluster (cluster A)and the South and Central American cluster (cluster B), were defined. Two subgroups, the Texas-California subgroup (subgroup Al) and the Missouri-Maryland-Florida subgroup (subgroup A2), were distinguished within group A. Similarly, group B strains were subclustered to a South American subgroup (subgroup B1) and a Central American subgroup (subgroup B2). These results were consistent with those obtained by DNA sequencing analysis. The ability ofthe BESS T-scan method to discriminate between strains that present with high degrees of nucleotide sequence similarity indicated that this method provides reliable results and multiple applications for other virus families. The method has proven to be suitable for phylogenetic comparison and molecular epidemiology studies and may be an alternative to DNA sequencing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:37:51