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Titolo:
TCR V-BETA USAGE BY ACETYLCHOLINE RECEPTOR-SPECIFIC CD4(-CELLS IN MYASTHENIA-GRAVIS() T)
Autore:
RAJU R; NAVANEETHAM D; PROTTI MP; HORTON RM; HOPPE BL; HOWARD J; CONTIFINE BM;
Indirizzi:
UNIV MINNESOTA,COLL BIOL SCI,DEPT BIOCHEM,1479 GORTNER AVE ST PAUL MN55108 UNIV MINNESOTA,COLL BIOL SCI,DEPT BIOCHEM ST PAUL MN 55108 UNIV MINNESOTA,SCH MED,DEPT PHARMACOL MINNEAPOLIS MN 55455 UNIV N CAROLINA,DEPT NEUROL CHAPEL HILL NC 27599
Titolo Testata:
Journal of autoimmunity
fascicolo: 2, volume: 10, anno: 1997,
pagine: 203 - 217
SICI:
0896-8411(1997)10:2<203:TVUBAR>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MYELIN BASIC-PROTEIN; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; EARLY RHEUMATOID-ARTHRITIS; MULTIPLE-SCLEROSIS; SYNOVIAL-FLUID; ALPHA-SUBUNIT; GENE USAGE; GAMMA-SUBUNIT; LYMPHOCYTES-T;
Keywords:
AUTOIMMUNITY; MYASTHENIA GRAVIS; PCR; PREFERENTIAL TCR V-BETA USAGE; T CELL EPITOPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
95
Recensione:
Indirizzi per estratti:
Citazione:
R. Raju et al., "TCR V-BETA USAGE BY ACETYLCHOLINE RECEPTOR-SPECIFIC CD4(-CELLS IN MYASTHENIA-GRAVIS() T)", Journal of autoimmunity, 10(2), 1997, pp. 203-217

Abstract

In myasthenia gravis the muscle acetylcholine receptor (AChR) is the target of an autoimmune response. AChR epitopes recognized by CD4(+) Tcells in myasthenic patients have been identified. AChR-specific CD4() cell lines can be propagated by stimulation of blood lymphocytes with synthetic or biosynthetic AChR sequences. We analysed, using a semi-quantitative PCR assay, the T cell receptor (TCR) V beta usage of 16 anti-AChR polyclonal CD4(+) T cell lines of known epitope specificity,propagated from myasthenic patients using pools of overlapping peptides corresponding to the sequence of an AChR subunit, or individual synthetic AChR sequences. Twelve lines had been propagated for less than 2 months, four lines for 3.5-5 months. Most lines had Limited V beta usage, but in most cases different V beta regions were used for different epitopes in the same patient, and for the same epitope in differentpatients. In a few patients, the same V beta regions were used for recognition of different epitopes. The V beta 4 and V beta 6 regions were used most frequently. These findings suggest that the potentially autoimmune T cells that survive clonal deletion have a limited TCR repertoire. Although the present data do allow conclusions on the role of asuperantigen in triggering the anti-AChR autoimmune response, the finding that different V beta regions were used in different patients does not support an important role of a superantigen in the maintenance of the CD4(+) response in myasthenia gravis. (C) 1997 Academic Press Limited.

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Documento generato il 28/09/20 alle ore 15:33:16