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Titolo:
PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis
Autore:
Mikosza, ASJ; La, T; Brooke, CJ; Lindboe, CF; Ward, PB; Heine, RG; Guccion, JG; de Boer, WB; Hampson, DJ;
Indirizzi:
Murdoch Univ, Div Vet & Biomed Sci, Murdoch, WA 6150, Australia Murdoch Univ Murdoch WA Australia 6150 d Sci, Murdoch, WA 6150, Australia Royaltraliarens Hosp, Dept Microbiol & Infect Dis, Parkville, Vic 3052, Aus Royal Childrens Hosp Parkville Vic Australia 3052 arkville, Vic 3052, Aus PathCtr, Dept Pathol Anat, Nedlands, WA 6009, Australia PathCtr Nedlands WA Australia 6009 hol Anat, Nedlands, WA 6009, Australia Vest Agder Cty Cent Hosp, Dept Pathol, N-4604 Kristiansand, Norway Vest Agder Cty Cent Hosp Kristiansand Norway N-4604 Kristiansand, Norway Dept Vet Affairs Med Ctr, Pathol & Lab Med Serv, Washington, DC USA Dept Vet Affairs Med Ctr Washington DC USA Med Serv, Washington, DC USA
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 6, volume: 37, anno: 1999,
pagine: 2093 - 2098
SICI:
0095-1137(199906)37:6<2093:PAFFTI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGEN-FREE CHICKS; SERPULINA-PILOSICOLI; SEQUENCE COMPARISONS; SP-NOV; HYODYSENTERIAE; IDENTIFICATION; PREVALENCE; INFECTION; DIARRHEA; BLOOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Hampson, DJ Murdoch Univ, Div Vet & Biomed Sci, Murdoch, WA 6150, Australia Murdoch Univ Murdoch WA Australia 6150 ch, WA 6150, Australia
Citazione:
A.S.J. Mikosza et al., "PCR amplification from fixed tissue indicates frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis", J CLIN MICR, 37(6), 1999, pp. 2093-2098

Abstract

PCR procedures amplifying portions of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Serpulina pilosicoli were applied to DNA extracted from paraffin-embedded human colonic or rectal tissues from 30 Norwegian, Australian, and U.S. patients, 16 of whom had histologic evidence of intestinal spirochetosis (IS). B. aalborgi-specific sequences were identified by PCR in 10 of the IS patients (62.5%) but none of the others, while S. pilosicoli sequences were not detected in tissues from any patient. Direct sequencing of products from three of the positive samples provided further confirmation of the presence of B. aalborgi. B. aalborgi may be a more commoncause of intestinal spirochetosis than has been previously thought.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:35:28