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Titolo:
The Drosophila Pkn protein kinase is a Rho Rac effector target required for dorsal closure during embryogenesis
Autore:
Lu, Y; Settleman, J;
Indirizzi:
Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA Massachusetts Gen Hosp Charlestown MA USA 02129 Charlestown, MA 02129 USA Harvard Univ, Sch Med, Charlestown, MA 02129 USA Harvard Univ CharlestownMA USA 02129 Sch Med, Charlestown, MA 02129 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 9, volume: 13, anno: 1999,
pagine: 1168 - 1180
SICI:
0890-9369(19990501)13:9<1168:TDPPKI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SHAPE CHANGES; SIGNAL-TRANSDUCTION PATHWAY; AMINO-TERMINAL KINASE; ACTIN STRESS FIBERS; CYTOSKELETAL ORGANIZATION; POTENTIAL EFFECTOR; SHEET MOVEMENT; MYOBLAST CITY; SMALL GTPASES; N PKN;
Keywords:
Pkn; Rho GTPase; Rac GTPase; dorsal closure; signal transduction; Drosophila;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Settleman, J Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA Massachusetts Gen Hosp Charlestown MA USA 02129 MA 02129 USA
Citazione:
Y. Lu e J. Settleman, "The Drosophila Pkn protein kinase is a Rho Rac effector target required for dorsal closure during embryogenesis", GENE DEV, 13(9), 1999, pp. 1168-1180

Abstract

The PKN family of PKC-related protein kinases constitutes the major Rho GTPase-associated protein kinase activities detected in mammalian tissues. However, the biological functions of these kinases are unknown. We have identified a closely related PKN homolog in Drosophila (Pkn) that binds specifically to GTP-activated Rho1 and Rac1 GTPases through distinct binding sites on Pkn. The interaction of Pkn with either of these GTPases results in increased kinase activity, suggesting that Pkn is a shared Rho/Rac effector target. Characterization of a loss-of-function mutant of Drosophila Pkn revealed that this kinase is required specifically for the epidermal cell shape changes during the morphogenetic process of dorsal closure of the developingembryo. Moreover, Pkn, as well as the Rho1 GTPase, mediate a pathway for cell shape changes in dorsal closure that is independent of the previously reported Rac GTPase-mediated Tun amino (N)-terminal kinase (JNK) cascade that regulates gene expression required for dorsal closure. Thus, it appears that distinct but coordinated Rho- and Rac-mediated signaling pathways regulate the cell shape changes required for dorsal closure and that Pkn provides a GTPase effector function for cell shape changes in vivo, which acts together with a Rac-JNK transcriptional pathway in the morphogenesis of the Drosophila embryo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 11:10:07