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Titolo:
The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
Autore:
Kang, YB; Cullen, BR;
Indirizzi:
Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 , Med Ctr, Dept Genet, Durham, NC 27710 USA Duke Univ, Med Ctr, Howard Hughes Med Inst, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 oward Hughes Med Inst, Durham, NC 27710 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 9, volume: 13, anno: 1999,
pagine: 1126 - 1139
SICI:
0890-9369(19990501)13:9<1126:THTPIA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; SIMIAN RETROVIRUS TYPE-1; REV ACTIVATION DOMAIN; PRE-MESSENGER-RNA; SECONDARY STRUCTURE; RESPONSE ELEMENT; TARGET SEQUENCE; HUMAN HOMOLOG; HNRNP A1; CYTOPLASM;
Keywords:
mRNA; nuclear export; nuclear import; retroviral; constitutive transport element;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Cullen, BR Duke Univ, Med Ctr, Dept Genet, Durham, NC 27710 USA Duke UnivDurham NC USA 27710 Dept Genet, Durham, NC 27710 USA
Citazione:
Y.B. Kang e B.R. Cullen, "The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences", GENE DEV, 13(9), 1999, pp. 1126-1139

Abstract

The constitutive transport element (CTE) encoded by simian type D retroviruses directs unspliced viral RNAs into a nuclear export pathway that is congruent with the pathway used by cellular mRNAs. Here, we show that quail cells are refractory to CTE function but become highly permissive upon expression of the human Tap protein, a candidate CTE cofactor. Tap contains a novel sequence-specific RNA binding domain that is sufficient for CTE binding but inadequate to support CTE function. Using microinjection assays, we have defined two NLSs and one NES in Tap. Mutational inactivation of the Tap NES, which lies outside the RNA-binding domain, not only blocks Tap functionbut also generates dominant-negative forms of Tap. Whereas replacement of the Tap NES with the well-defined Rev NES rescues the ability of Tap to support CTE function, this substitution also confers sensitivity to agents that block the activity of Crm1, the Rev NES cofactor. Together, these data validate Tap as the first human sequence-specific nuclear mRNA export factor and identify a novel type of NES that can support nuclear mRNA export but does not act via Crm1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:33:33