Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Fas-activated apoptosis and apoptosis mediators in human trabecular meshwork cells
Autore:
Agarwal, R; Talati, M; Lambert, W; Clark, AF; Wilson, SE; Agarwal, N; Wordinger, RJ;
Indirizzi:
Univ N Texas, Hlth Sci Ctr, Dept Cell Biol & Anat, Ft Worth, TX 76107 USA Univ N Texas Ft Worth TX USA 76107 ll Biol & Anat, Ft Worth, TX 76107 USA Univ N Texas, Hlth Sci Ctr, N Texas Eye Res Inst, Ft Worth, TX 76107 USA Univ N Texas Ft Worth TX USA 76107 s Eye Res Inst, Ft Worth, TX 76107 USA Alcoa Labs, Glaucoma Res, Ft Worth, TX USA Alcoa Labs Ft Worth TX USAAlcoa Labs, Glaucoma Res, Ft Worth, TX USA Univ Washington, Sch Med, Dept Ophthalmol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 pt Ophthalmol, Seattle, WA 98195 USA
Titolo Testata:
EXPERIMENTAL EYE RESEARCH
fascicolo: 5, volume: 68, anno: 1999,
pagine: 583 - 590
SICI:
0014-4835(199905)68:5<583:FAAAMI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR; DEATH; GENE; EXPRESSION; LIGAND; CELLULARITY; RECEPTORS; ENZYME; CORNEA;
Keywords:
apoptosis; glaucoma; trabecular meshwork; Fas; Fas ligand; Bcl-2; Bcl-xl; ICE; Bax;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Wordinger, RJ Univ N Texas, Hlth Sci Ctr, Dept Cell Biol & Anat, 3500 CampBowie Blvd, Ft Univ N Texas 3500 Camp Bowie Blvd Ft Worth TX USA 76107 , Ft
Citazione:
R. Agarwal et al., "Fas-activated apoptosis and apoptosis mediators in human trabecular meshwork cells", EXP EYE RES, 68(5), 1999, pp. 583-590

Abstract

A gradual loss of cells occurs within the human trabecular meshwork duringnormal aging and appears to be increased in patients with primary open-angle glaucoma. The exact mechanism by which cells are lost in either condition is not known, however phagocytosis, cell migration and cell death have been suggested. Apoptosis is one method by which cell death can occur. We have examined the modulators for apoptosis within the human trabecular meshwork using both cell lines and ex-vivo dissected trabecular meshwork tissues obtained from normal donors. Using RT-PCR it was shown that mRNA for severalmodulators of apoptosis (Fas, Bcl-2, Bcl-xl, Pax, and ICE) are expressed by both cell lines and ex-vivo tissues. Apoptosis was stimulated to occur bytreating cell lines with a monoclonal antibody (IgM) to Pas. Apoptosis wasverified via morphological changes to the cells, transferase-mediated dUTPnick-end labeling TUNEL Immunofluorescence, and DNA laddering. Control cells exposed to IgM did not undergo apoptosis. These results represent the first report of apoptosis modulators within the human trabecular meshwork anddemonstrate that human trabecular meshwork cells can be stimulated to undergo apoptosis via the Fas/FasL pathway. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:33:42