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Titolo:
Megakaryocytic growth in patients with refractory anemia is suppressed by treatment with interferon alpha
Autore:
Hofmann, WK; Kalina, U; Seipelt, G; Hoffmann, K; Wagner, S; Hoelzer, D; Ottmann, OG;
Indirizzi:
Univ Frankfurt Klinikum, Dept Haematol, D-60590 Frankfurt, Germany Univ Frankfurt Klinikum Frankfurt Germany D-60590 590 Frankfurt, Germany Univ Jena Hosp, Dept Haematol & Oncol, Jena, Germany Univ Jena Hosp JenaGermany Hosp, Dept Haematol & Oncol, Jena, Germany
Titolo Testata:
EUROPEAN JOURNAL OF HAEMATOLOGY
fascicolo: 5, volume: 62, anno: 1999,
pagine: 336 - 340
SICI:
0902-4441(199905)62:5<336:MGIPWR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TRANS-RETINOIC ACID; MYELODYSPLASTIC SYNDROMES; COMBINATION THERAPY; PROGENITOR CELLS; LEUKEMIA; DIFFERENTIATION; HEMATOPOIESIS; INDUCTION; TRIAL;
Keywords:
interferon alpha; megakaryocytes; myelodysplastic syndrome; PEG-rHuMGDF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Hofmann, WK Univ Frankfurt Klinikum, Dept Haematol, D-60590 Frankfurt, Germany Univ Frankfurt Klinikum Frankfurt Germany D-60590 t, Germany
Citazione:
W.K. Hofmann et al., "Megakaryocytic growth in patients with refractory anemia is suppressed by treatment with interferon alpha", EUR J HAEMA, 62(5), 1999, pp. 336-340

Abstract

IFN alpha alone or in combination with retinoids or haematopoietic growth factors has been used to treat patients with early MDS because of its properties as a differentiation inducing agent. We investigated whether treatment of patients with refractory anemia (RA) with IFN alpha (1.5x10(6) IU twice a week) and intermittent all-trans retinoic acid (ATRA, 25 mg/m(2)/d) influences in-vitro megakaryocytic (MK) proliferation and differentiation stimulated by PEG-rHuMGDF. Low density non-adherent bone marrow (BM) cells from8 patients with RA were assayed prior to any treatment other than supportive and after a period of 6 months of treatment. MK development was assayed in suspension cultures in the presence of PEG-rHuMGDF and SCF for 7 d usingmorphological criteria and flowcytometric analysis of CD42b (GP1b) positive cells. BM-cells from 10 healthy individuals served as control. Following stimulation with PEG-rHuMGDF 23+/-7% and 16+/-4% of control cells were CD42b positive after 5 and 7 d of cultures, respectively. In cultures of cells from MDS patients prior to treatment 8+/-2% and 7+/-3% of cells were CD42bon days 5 and 7. In the course of IFN alpha treatment cultures of all BM samples from these MDS patients revealed a significant reduction of MK precursor cells (3+/-2% CD42b+, p=0.03 and 0.04). In conclusion, treatment with IFN alpha and ATRA did not result in improved megakaryocytopoiesis as assessed by in-vitro cultures. On the contrary, low-dose IFN alpha appears to suppress cell proliferation as well as MK development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/11/18 alle ore 04:22:38