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Titolo:
Relationships between muscle mitochondrial DNA content, mitochondrial enzyme activity and oxidative capacity in man: alterations with disease
Autore:
Wang, H; Hiatt, WR; Barstow, TJ; Brass, EP;
Indirizzi:
Univ Calif Los Angeles, Harbor Med Ctr, Dept Med, Torrance, CA 90509 USA Univ Calif Los Angeles Torrance CA USA 90509 Med, Torrance, CA 90509 USA Univ Colorado, Hlth Sci Ctr, Denver, CO USA Univ Colorado Denver CO USAUniv Colorado, Hlth Sci Ctr, Denver, CO USA Colorado Prenvent Ctr, Denver, CO USA Colorado Prenvent Ctr Denver CO USA olorado Prenvent Ctr, Denver, CO USA Kansas State Univ, Dept Kinesiol, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 inesiol, Manhattan, KS 66506 USA
Titolo Testata:
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY
fascicolo: 1, volume: 80, anno: 1999,
pagine: 22 - 27
SICI:
0301-5548(199906)80:1<22:RBMMDC>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKELETAL-MUSCLE; PERIPHERAL ARTERIAL INSUFFICIENCY; INTERMITTENT CLAUDICATION; ELECTRICAL-STIMULATION; CONTRACTILE ACTIVITY; LACTIC-ACIDOSIS; GENE-EXPRESSION; OXYGEN-UPTAKE; EXERCISE; ADAPTATION;
Keywords:
mitochondria; exercise; mitochondrial DNA; peripheral arterial disease; paraplegia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Brass, EP Univ,Calif Los Angeles, Harbor Med Ctr, Dept Med, 1000 W Carson St Torrance Univ Calif Los Angeles 1000 W Carson St Torrance Torrance CA USA 90509
Citazione:
H. Wang et al., "Relationships between muscle mitochondrial DNA content, mitochondrial enzyme activity and oxidative capacity in man: alterations with disease", EUR J A PHY, 80(1), 1999, pp. 22-27

Abstract

Muscle mitochondrial content is tightly regulated, and requires the expression of both nuclear and mitochondrial genes. In addition, muscle mitochondrial content is a major determinant of aerobic exercise capacity in healthysubjects. The current study was designed to test the hypothesis that in healthy humans, muscle mitochondrial DNA (mtDNA) content is correlated with citrate synthase activity (a representative nuclear-encoded mitochondrial enzyme) and aerobic exercise capacity as defined by whole-body peak oxygen consumption ((V) over dot O-2). Furthermore, it was postulated that these relationships might be altered with disease. Twelve I; healthy and five paraplegic subjects underwent exercise testing and vastus lateralis muscle biopsysampling. An additional ten healthy subjects and eight patients with unilateral peripheral arterial disease (PAD) underwent exercise testing and gastrocnemius muscle biopsy sampling. Citrate synthase activity and mtDNA content were positively correlated in the vastus lateralis muscles from the healthy subjects. This relationship was similar in muscle from paraplegic subjects. mtDNA content was positively correlated with peak (V) over dot O-2 in the healthy subjects and in the paraplegic subjects in whom peak (V) over dot O-2 had been elicited by functional electrical stimulation of the muscle. In contrast, the PAD subjects demonstrated higher mtDNA contents than would have been predicted based on their claudication-limited peak (V) over dot O-2 Thus, in healthy humans there are strong relationships between musclemtDNA content and both muscle citrate synthase activity and peak (V) over dot O-2. These relationships are consistent with coordinant nuclear DNA andmtDNA expression, and with mitochondrial content being a determinant of aerobic exercise capacity. The relationships seen in healthy humans are quantitatively similar in paraplegic patients, but not in patients with PAD, a disease which is associated with a metabolic myopathy. The relationships between mtDNA content, mitochondrial enzyme activities and exercise capacity provide insight into the physiologic and pathophysiologic regulation of muscle mitochondrial expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 11:22:32