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Titolo:
Establishment of a simple and practical procedure applicable to therapeutic angiogenesis
Autore:
Kanno, S; Oda, N; Abe, M; Saito, S; Hori, K; Handa, Y; Tabayashi, K; Sato, Y;
Indirizzi:
Tohoku Univ, Inst Dev Aging & Canc, Dept Vasc Biol, Aoba Ku, Sendai, Miyagi Tohoku Univ Sendai Miyagi Japan 9808575 asc Biol, Aoba Ku, Sendai, Miyagi Tohokuai,iv, Grad Sch Med, Dept Restorat Neuromuscular Surg & Rehabil, Send Tohoku Univ Sendai Miyagi Japan 980 at Neuromuscular Surg & Rehabil, Send Tohokupaniv, Sch Med, Dept Thorac & Cardiovasc Surg, Sendai, Miyagi 980, Ja Tohoku Univ Sendai Miyagi Japan 980 rdiovasc Surg, Sendai, Miyagi 980, Ja
Titolo Testata:
CIRCULATION
fascicolo: 20, volume: 99, anno: 1999,
pagine: 2682 - 2687
SICI:
0009-7322(19990525)99:20<2682:EOASAP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOTHELIAL GROWTH-FACTOR; TERM ELECTRICAL-STIMULATION; RABBIT FAST MUSCLES; BLOOD-FLOW; CAPILLARY DENSITY; LIMB ISCHEMIA; GENE-TRANSFER; EXPRESSION; MYOCARDIUM; ACTIVATION;
Keywords:
electrical stimulation; growth substances; angiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, Y Tohokui,niv, Inst Dev Aging & Canc, Dept Vasc Biol, Aoba Ku, 4-1 Seiryomach Tohoku Univ 4-1 Seiryomachi Sendai Miyagi Japan 9808575 Seiryomach
Citazione:
S. Kanno et al., "Establishment of a simple and practical procedure applicable to therapeutic angiogenesis", CIRCULATION, 99(20), 1999, pp. 2682-2687

Abstract

Background-Therapeutic angiogenesis is thought to be beneficial For serious ischemic diseases. This investigation was designed to establish a simple and practical procedure applicable to therapeutic angiogenesis. Methods and Results-When cultured skeletal muscle cells were electrically stimulated at a voltage that did not cause their contraction, vascular endothelial growth factor (VEGF) mRNA was augmented at an optimal-frequency stimulation. This increase of VEGF mRNA was derived primarily from transcriptional activation. Electrical stimulation increased the secretion of VEGF protein into the medium. This conditioned medium then augmented the growth of endothelial cells. The effect of electrical stimulation was further confirmed in a rat model of hindlimb ischemia. The tibialis anterior muscle in theischemic limb was electrically stimulated. The frequency of stimulation was 50 Hz and strength was 0.1 V, which was far below the threshold for muscle contraction. After a 5-day stimulation, there was a significant increase in blood flow within the muscle. Immunohistochemical analysis revealed thatVEGF protein was synthesized and capillary density was significantly increased in the stimulated muscle. Rats tolerated this procedure very well, andthere was no muscle contraction, muscle injury, or restriction in movement. Conclusions-We propose this procedure as a simple and practical method of therapeutic angiogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:26:02