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Titolo:
Resistance to apoptosis in CTLL-2 cells overexpressing B-Myb is associatedwith B-Myb-dependent bcl-2 induction
Autore:
Grassilli, E; Salomoni, P; Perrotti, D; Franceschi, C; Calabretta, B;
Indirizzi:
Thomas Jefferson Univ, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Thomas Jefferson Univ, Kimmel Canc Ctr, Philadelphia, PA 19107 USA Thomas Jefferson Univ Philadelphia PA USA 19107 hiladelphia, PA 19107 USA Univ Modena, Dept Biomed Sci, Sect Gen Pathol, I-41100 Modena, Italy Univ Modena Modena Italy I-41100 Sect Gen Pathol, I-41100 Modena, Italy
Titolo Testata:
CANCER RESEARCH
fascicolo: 10, volume: 59, anno: 1999,
pagine: 2451 - 2456
SICI:
0008-5472(19990515)59:10<2451:RTAICC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING ACTIVITY; C-MYB; CONSTITUTIVE EXPRESSION; MYELOID CELLS; T-CELLS; PROTEIN; ACTIVATION; DIFFERENTIATION; DEATH; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Calabretta, B Thomas,Jefferson Univ, Dept Microbiol & Immunol, 233S 10th St, Philadelphia Thomas Jefferson Univ 233S 10th St Philadelphia PA USA 19107
Citazione:
E. Grassilli et al., "Resistance to apoptosis in CTLL-2 cells overexpressing B-Myb is associatedwith B-Myb-dependent bcl-2 induction", CANCER RES, 59(10), 1999, pp. 2451-2456

Abstract

Transcriptional regulators of the Myb family play important roles in cell proliferation, differentiation, and survival. To investigate the role of Myb proteins in the regulation of apoptosis, we studied the apoptotic response of interleukin 2-dependent CTLL-2 cells stably transfected with B-Myb. B-Myb-overexpressing cells showed a diminished cytokine dependence and were resistant to apoptosis induced by doxorubicin, ceramide, and dexamethasone, Overexpression of B-Myb was associated with enhanced expression of bcl-2, which was dependent, at least in part, on increased transcription. In transient transfection assays in T-lymphoblastic cells, B-Myb was able to stimulate the promoter activity of the bcl-2 5' flanking region linked to the chloramphenicol acetyltransferase reporter gene. A segment of the bcl-2 promoter (nucleotides +34 to +58 relative to the transcription initiation site) contained a putative Myb-binding site and was shown to specifically interact with B-Myb and to confer B-Myb responsiveness to a bcl-2/chloramphenicol acetyltransferase reporter construct. These results indicate that B-Myb promotes T cells survival by enhancing the expression of bcl-2 and identify bcl-2 as a B-Myb target gene regulated in a DNA binding-dependent manner.

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Documento generato il 01/10/20 alle ore 07:39:33