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Titolo:
C-terminal region of hTPO is important for secretion and expression in insect cells
Autore:
Ahn, HK; Chung, JY; Park, SK; Joo, SM; Park, SK; Koh, YW;
Indirizzi:
Daewoong Pharmaceut Co Ltd, Ctr Res & Dev, Sunhnam 462120, South Korea Daewoong Pharmaceut Co Ltd Sunhnam South Korea 462120 62120, South Korea
Titolo Testata:
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
fascicolo: 5, volume: 47, anno: 1999,
pagine: 729 - 733
SICI:
1039-9712(199905)47:5<729:CROHII>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
MPL LIGAND; THROMBOPOIETIN; GLYCOSYLATION; STIMULATION; CLONING;
Keywords:
truncated hTPO; secretion; expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Koh, YW Daewoong Pharmaceut Co Ltd, Ctr Res & Dev, Sunhnam 462120, South Korea Daewoong Pharmaceut Co Ltd Sunhnam South Korea 462120 outh Korea
Citazione:
H.K. Ahn et al., "C-terminal region of hTPO is important for secretion and expression in insect cells", BIOC MOL B, 47(5), 1999, pp. 729-733

Abstract

Human thrombopoietin (hTPO) variant cDNAs truncated in the C-terminal regions of wild-type hTPO (332 amino acids) were constructed by PCR and expressed in Trichoplusia ni (Tn5) insect cells using a baculovirus expression system. Each variant, hTPO163 (amino acids 1-163), hTPO198 (1-198) and hTPO245(1-245), was produced in insect cells with very low efficiency in comparison with wild-type hTPO. Immunoblot analysis showed that the predicted 20, 25 and 34 kDa molecular sizes corresponding to hTPO163, hTPO198 and hTPO245,respectively, were barely detected in culture medium and most of the proteins remained within the cell. These results suggest that C-terminal regionscontaining potential N-glycosylation sites of hTPO are required for the secretion of hTPO into culture medium as well as expression in insect cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:19:28