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Titolo:
Purification and characterization of a beta-glucosidase specific for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) glucoside in maize
Autore:
Oikawa, A; Ebisui, K; Sue, M; Ishihara, A; Iwamura, H;
Indirizzi:
Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 , Div Appl Life Sci, Kyoto 6068502, Japan
Titolo Testata:
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
fascicolo: 3-4, volume: 54, anno: 1999,
pagine: 181 - 185
SICI:
0939-5075(199903/04)54:3-4<181:PACOAB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROXAMIC ACIDS; DEFENSE; WHEAT;
Keywords:
benzoxazinones; Zea mays; gramineae; beta-glucosidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Iwamura, H Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Life Sci, Kyoto 6068502, Japan
Citazione:
A. Oikawa et al., "Purification and characterization of a beta-glucosidase specific for 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) glucoside in maize", Z NATURFO C, 54(3-4), 1999, pp. 181-185

Abstract

Occurrence and properties of hydroxamic acid glucoside glucosidase were investigated in 10-day-old, autotrophic maize (Zea mays L.) in which 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-G) is a major benzoxazinone component. Crude ex tracts of both leaves and roots showed glucosidase activity for both DIMBOA-G and 2,4dihydroxy-1,4-benzoxazin-3-one glucoside (DLBOA-G). A cation-exchange chromatography after cryoprecipitation of the extract from leaves gave a peak with both activities, and further purification by ion-exchange and hydroxyapatite chromatography gave a fraction with an apparent homogeneity, the purification being 560 fold. The K-m values (mM) of the purified glucosidase were 0.16 for DIMBOA-G, 0.68 for DIBOA-Gand 2.96 for p-nitrophenyl-beta-D-glucopyranoside. The activity on salicinand esculin was too low to be detected. The data indicate that a glucosidase specific for DIMBOA-G comes into contact with constitutive benzoxazinoneglucosides producing defensive aglycone when plants are damaged by microbial or insect attacks.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:38:49