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Titolo:
Activation and repression of transcription by auxin-response factors
Autore:
Ulmasov, T; Hagen, G; Guilfoyle, TJ;
Indirizzi:
Univ Missouri, Dept Biochem, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 Dept Biochem, Columbia, MO 65211 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 10, volume: 96, anno: 1999,
pagine: 5844 - 5849
SICI:
0027-8424(19990511)96:10<5844:AAROTB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOYBEAN GH3 PROMOTER; PROTEIN-PROTEIN INTERACTIONS; CONSERVED B3 DOMAIN; DNA-BINDING; AUX/IAA PROTEINS; GENE-EXPRESSION; ARF FAMILY; ELEMENTS; VIVIPAROUS1; CONTAINS;
Keywords:
transcriptional activators; dimerization domain; AuxRE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Guilfoyle, TJ 800 N Lindberg Blvd,Mail Zone U3E, St Louis, MO 63167 USA 800 N Lindberg Blvd,Mail Zone U3E St Louis MO USA 63167 USA
Citazione:
T. Ulmasov et al., "Activation and repression of transcription by auxin-response factors", P NAS US, 96(10), 1999, pp. 5844-5849

Abstract

Auxin-response factors (ARFs) bind with specificity to TGTCTC auxin-response elements (AuxREs), which are found in promoters of primary/early auxin-response genes. Nine different ARFs have been analyzed for their capacity toactivate or repress transcription in transient expression assays employingauxin-responsive GUS reporter genes. One ARF appears to act as a repressor, Four ARFs function as activators and contain glutamine-rich activation domains. To achieve transcriptional activation on TGTCTC AuxREs in transient expression assays, ARFs require a conserved dimerization domain found in both ARF and Aux/IAA proteins, but they do not absolutely require their DNA-binding domains. Our results suggest that ARFs can activate or repress transcription by binding to AuxREs directly and that selected ARFs, when overexpressed, may potentiate activation further by associating with an endogenoustranscription factor(s) (e.g., an ARF) that is bound to AuxREs. Transfection experiments suggest that TGTCTC AuxREs are occupied regardless of the auxin status in cells and that these occupied AuxREs are activated when exogenous auxin is applied to cells or when ARF activators are overexpressed, The results provide new insight into mechanisms involved with auxin regulation of primary/early-response genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:44:20