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Titolo:
Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides: differences in the signal transduction pathways leading to the activation of elicitor-responsive genes
Autore:
Nishizawa, Y; Kawakami, A; Hibi, T; He, DY; Shibuya, N; Minami, E;
Indirizzi:
Natlpanst Agrobiol Resources, Dept Biotechnol, Tsukuba, Ibaraki 3058602, Ja Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 ki 3058602, Ja
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 5, volume: 39, anno: 1999,
pagine: 907 - 914
SICI:
0167-4412(199903)39:5<907:ROTCGE>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-AFFINITY BINDING; PHENYLALANINE AMMONIA-LYASE; PHYTOALEXIN PRODUCTION; MEMBRANE DEPOLARIZATION; RHIZOCTONIA-SOLANI; PLASMA-MEMBRANE; OXIDATIVE BURST; BIOTIC ELICITOR; ION FLUXES; INDUCTION;
Keywords:
chitin oligomer; chitinase; elicitor; gene expression; rice; signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Nishizawa, Y Natlaraki Agrobiol Resources, Dept Biotechnol, 2-1-2 Kannondai, Tsukuba, Ib Natl Inst Agrobiol Resources 2-1-2 Kannondai Tsukuba IbarakiJapan 3058602
Citazione:
Y. Nishizawa et al., "Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharides: differences in the signal transduction pathways leading to the activation of elicitor-responsive genes", PLANT MOL B, 39(5), 1999, pp. 907-914

Abstract

Expression patterns of chitinase transcripts induced by N-acetylchitooligosaccharide elicitor were analyzed by northern blot hybridization in order to reveal a signal transduction pathway leading to the activation of class Ichitinase genes (Cht-1 and Cht-3), which may play an important role in producing N-acetylchitooligosaccharide elicitor. The transcription level of both genes was enhanced in response to N-acetylchitooligosaccharides larger than pentaose at subnanomolar concentrations. These structure and dose dependencies were consistent not only with those for a 75 kDa high-affinity binding protein for N-acetylchitooligosaccharide elicitor in the plasma membrane, but also with other series of cellular responses including phytoalexin production and the expression of elicitor-responsive genes (EL2, EL3). Therefore, the elicitor signal to evoke these cellular responses including the activation of the chitinase genes could be common and transmitted into cellsthrough the 75 kDa protein. However, the signal transduction pathway for the activation of the chitinase gene appeared to diverge from those for the other elicitor-responsive genes shortly after the signal perception. It wasshown that the induction of chitinase expression by N-acetylchitooligosaccharide would require protein phosphorylation, but not de novo protein synthesis. The oxidative burst was demonstrated not to be necessary for transcriptional induction of the all four elicitor-responsive genes (Cht, PAL, EL2,EL3) by N-acetylchitooligosaccharide.

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Documento generato il 21/09/20 alle ore 05:35:25