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Titolo:
Expression and physical association of Fc alpha receptor and Fc receptor gamma chain in human mesangial cells
Autore:
Suzuki, Y; Ra, C; Saito, K; Horikoshi, S; Hasegawa, S; Tsuge, T; Okumura, K; Tomino, Y;
Indirizzi:
Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 113, Japan JuntendoUniv Tokyo Japan 113 Dept Immunol, Bunkyo Ku, Tokyo 113, Japan Juntendo Univ, Sch Med, Div Nephrol, Dept Med, Tokyo 113, Japan Juntendo Univ Tokyo Japan 113 d, Div Nephrol, Dept Med, Tokyo 113, Japan
Titolo Testata:
NEPHROLOGY DIALYSIS TRANSPLANTATION
fascicolo: 5, volume: 14, anno: 1999,
pagine: 1117 - 1123
SICI:
0931-0509(199905)14:5<1117:EAPAOF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
IGA NEPHROPATHY; EPSILON-RI; CELLULAR-DISTRIBUTION; TRANSFECTED CELLS; POLYMERIC IGA; IN-VITRO; CD89; RAT; GLOMERULONEPHRITIS; SUBUNIT;
Keywords:
Fc alpha R; FcR gamma chain; IgA nephropathy; mesangial cells; physical association;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Ra, C Juntendopaniv, Sch Med, Dept Immunol, Bunkyo Ku, 2-1-1 Hongo, Tokyo 113, Ja Juntendo Univ 2-1-1 Hongo Tokyo Japan 113 -1-1 Hongo, Tokyo 113, Ja
Citazione:
Y. Suzuki et al., "Expression and physical association of Fc alpha receptor and Fc receptor gamma chain in human mesangial cells", NEPH DIAL T, 14(5), 1999, pp. 1117-1123

Abstract

Background. Most intensive investigations on the pathogenesis of IgA nephropathy have focused on the process before IgA deposition and the characteristics of IgA/IgA immune complex (IgA IC), but it still remains uncertain whether mesangial IgA ICs may cause glomerular injuries directly or are only secondary events of another pathological process. To assess the role of IgAICs in IgA nephropathy, we investigated the characteristics of Fc alpha receptor (Fc alpha R) and FcR gamma chain which is a signalling subunit of FcR in human mesangial cells (MCs). Methods. Gene expression of Fc alpha R and FcR gamma chain of human cultured MCs was examined by RT-PCR and subsequent Southern blot analyses. Sequence analyses after subcloning were also performed for further confirmation. Expression of Fc alpha R and FcR gamma chain at the protein level and theirphysical association in MCs were determined by immunoprecipitation after stimulation of the cells with heat-aggregated IgA. Results. Two distinct cDNA products were amplified from each cultured MC line. The sequence of the major product of similar to 900 bp was completely identical to that of Fc alpha R previously described. The smaller product had a 288 bp deletion which corresponded to exon 2 encoding the extracellular domain 2 of Fc alpha R. Gene expression of FcR gamma chain was also confirmed. Furthermore, we proved the physical association of Fc alpha R with the FcR gamma chain by co-immunoprecipitation under stimulation with a high dose of the heat-aggregated IgA. Conclusion. These findings suggested that polymeric IgA and/or IgA IC can directly activate MCs via Fc alpha R associated with the gamma chain. Our data also indicated that phenotypic variations of Fc alpha R occur on MC, such as splicing forms, the gamma chain association and/or the a chain expression itself, which may contribute to the pathogenesis of IgA nephropathy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:26:49