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Titolo:
Hepatocyte growth inhibitory factor derived from HTLV-I(+) T-cell line is identical to IL-6
Autore:
Kawai, Y; Yamauchi, A; Nakamura, H; Nakamura, Y; Hirose, T; Tsuyuki, S; Shinkura, N; Okawa, K; Iwamatsu, A; Maeda, Y; Ikai, I; Yamaoka, Y; Inamoto, T;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Surg Gastroenterol, Sakyo Ku, Kyoto 6068507, Kyoto Univ Kyoto Japan 6068507 g Gastroenterol, Sakyo Ku, Kyoto 6068507, Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanagawa, Japan Kirin BreweryCo Ltd Kanagawa Japan t Labs Key Technol, Kanagawa, Japan Kinki Univ, Sch Med, Dept Internal Med 3, Osaka 589, Japan Kinki Univ Osaka Japan 589 ch Med, Dept Internal Med 3, Osaka 589, Japan Kyoto Univ, Coll Med Technol, Kyoto 606, Japan Kyoto Univ Kyoto Japan 606 yoto Univ, Coll Med Technol, Kyoto 606, Japan
Titolo Testata:
LEUKEMIA RESEARCH
fascicolo: 5, volume: 23, anno: 1999,
pagine: 489 - 497
SICI:
0145-2126(199905)23:5<489:HGIFDF>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHASE PROTEIN RESPONSE; ADULT-RAT HEPATOCYTES; STIMULATING FACTOR; PRIMARY CULTURE; LIVER-FAILURE; DNA-SYNTHESIS; FACTOR-ALPHA; INTERLEUKIN-6; PROLIFERATION; INVOLVEMENT;
Keywords:
hepatocyte; inhibitor; HTLV-I; T-cell line; IL-6; ATL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Yamauchi, A Kyotoaraiv, Grad Sch Med, Dept Surg Gastroenterol, Sakyo Ku, 54 Shogoin Kaw Kyoto Univ 54 Shogoin Kawara Cho Kyoto Japan 6068507 goin Kaw
Citazione:
Y. Kawai et al., "Hepatocyte growth inhibitory factor derived from HTLV-I(+) T-cell line is identical to IL-6", LEUK RES, 23(5), 1999, pp. 489-497

Abstract

We previously reported that the culture supernatant of the human T-cell leukemia virus (HTLV-I) infected-T-cell line-ATL2-included factor(s), which had an inhibitory effect on epidermal growth factor (EGF)-stimulated proliferation of primary cultured rat hepatocytes. After crude purification, we arbitrarily named it hepatocyte growth inhibitory factor (HGI). In this study, we further purified HGI and determined its amino acid sequence. For purification, we used 4-steps column chromatography and SDS-PAGE. The purified proteins consisted of two bands of 20 and 27 kDa in SDS-PAGE analysis. Protein extracted from each band had an inhibitory effect on rat hepatocyte growth. Amino acid analysis of the purified 20 kDa band revealed that the 34 amino acids were identical to those of IL-6. The inhibitory effect of the factor was neutralized by an anti IL-6 neutralizing antibody. Using Western blot analysis of HGI, an anti IL-6 antibody recognized both 20 and 27 kDa bands. Consequently HGI was determined to be identical to IL-6, which occurredin higher levels in the sera of adult T-cell leukemia (ATL) patients. (C) 1999 Elsevier Science Ltd. All rights reserved.

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Documento generato il 15/01/21 alle ore 23:11:34