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Titolo:
The proliferative response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model
Autore:
Minion, DJ; van de Kerkhove, MP; Goodman, GR; van Aalst, JA; Absood, A; Fox, PL; Graham, LM;
Indirizzi:
Case Western Reserve Univ, Dept Surg, Cleveland, OH 44106 USA Case WesternReserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Vet Affairs Med Ctr, Cleveland, OH USA Vet Affairs Med Ctr Cleveland OH USA Affairs Med Ctr, Cleveland, OH USA ClevelandAClin Fdn, Lerner Res Inst, Dept Cell Biol, Cleveland, OH 44195 US Cleveland Clin Fdn Cleveland OH USA 44195 ll Biol, Cleveland, OH 44195 US Univ Michigan, Hlth Syst, Dept Surg, Ann Arbor, MI USA Univ Michigan Ann Arbor MI USA , Hlth Syst, Dept Surg, Ann Arbor, MI USA Vet Affairs Med Ctr, Ann Arbor, MI USA Vet Affairs Med Ctr Ann Arbor MI USA Affairs Med Ctr, Ann Arbor, MI USA
Titolo Testata:
JOURNAL OF VASCULAR SURGERY
fascicolo: 5, volume: 29, anno: 1999,
pagine: 845 - 850
SICI:
0741-5214(199905)29:5<845:TPRTPG>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DACRON GRAFTS; EXPRESSION; INJURY; INVIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Minion, DJ UnivUSAntucky, Albert B Chandler Med Ctr, 800 Rose St, Lexington, KY 40536 Univ Kentucky 800 Rose St Lexington KY USA 40536 gton, KY 40536
Citazione:
D.J. Minion et al., "The proliferative response to platelet-derived growth factor of smooth muscle cells isolated from synthetic vascular grafts in a canine model", J VASC SURG, 29(5), 1999, pp. 845-850

Abstract

Objective: Previous studies on graft healing have shown increased platelet-derived growth factor (PDGF) production in graft segments versus native aortic segments. The purpose of this study was to characterize the proliferative response of graft smooth muscle cells (SMCs) to PDGF. Methods: Thoracoabdominal grafts were implanted in beagles. SMCs were harvested from the graft and the proximal and distal aortas. Basal proliferation was assessed with growth curves in primary culture. The proliferative response to PDGF then was compared with [H-3]thymidine uptake studies and cellcounts. finally, PDGF receptors were characterized with radio-labeled ligand binding assays. Results: The growth curves showed that the graft SMCs entered log-phase growth 2 days earlier than did the aortic SMCs. Stimulation of quiescent early-passage graft SMCs with PDGF (10 ng/ml;) resulted in a 1.7 +/- 0.1-fold increase in [H-3]thymidine incorporation, which was significantly less than that of the SMCs fi om both the proximal aorta (11.8 +/- 3.0) and the distal aorta (10.2 +/- 1.9; P < .5). Similarly, the 1.1 +/- 0.1-fold increase ingraft SMC cell number was significantly less than the increases for both proximal (2.8 +/- 0.5) and distal (2.9 +/- 0.8) aortic SMCs (P < .5). Binding studies on quiescent first-passage cells showed fewer PDGF receptors available for binding in the graft SMCs (185 +/- 70 fmol/million cells) as compared with both the proximal (419 +/- 147 fmol/million cells) and the distal(387 +/- 112 fmol/million cells) aortas (P < .5). Binding affinity was similar for the three groups. Conclusion: Graft SMCs exist in a chronic proliferative state but exhibit a decreased proliferative response to PDGF and have fewer receptors available for binding PDGF than do aortic SMCs in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 03:13:20