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Titolo:
Exocytotic protein components in rat pituitary gland after long-term estrogen administration
Autore:
Majo, G; Lorenzo, MJ; Blasi, J; Aguado, F;
Indirizzi:
UnivlBarcelona, Bellvitge Hosp, Fac Med,Dept Biol Cellular & Anat Patol, Mo Univ Barcelona Lhospitalet De Llobregat Spain 08907 lar & Anat Patol, Mo Univ Alcala de Henares, Dept Bioquim & Biol Mol, E-28871 Alcala De Henares, Univ Alcala de Henares Alcala De Henares Spain E-28871 lcala De Henares,
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 2, volume: 161, anno: 1999,
pagine: 323 - 331
SICI:
0022-0795(199905)161:2<323:EPCIRP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMOLYTIC PLAQUE-ASSAY; ANTERIOR-PITUITARY; REGULATED EXOCYTOSIS; ENDOCRINE-CELLS; SYNAPTOTAGMIN-I; GROWTH-HORMONE; MESSENGER-RNAS; SNAP RECEPTORS; SECRETION; FUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Aguado, F UnivCBarcelona, Fac Med, Dept Biol Cellular & Anat Patol, CampusBellvitge, Univ Barcelona Campus Bellvitge,C Feixa Llarga S-N Lhospitalet De Llobregat Spain 08907
Citazione:
G. Majo et al., "Exocytotic protein components in rat pituitary gland after long-term estrogen administration", J ENDOCR, 161(2), 1999, pp. 323-331

Abstract

Recently, a set of proteins involved in the docking and fusion machinery of secretory organelles has been identified in anterior pituitary cells. In this study we analyzed, by Western blotting and immunocytochemistry, the expression of several proteins involved in exocytosis after long-term administration of 17 beta-estradiol (E-2) in Fischer 344 rats. No differences wereobserved in the amount of synaptosomal-associated protein of 25 kDa, synaptobrevin 2, syntaxin 1, synaptotagmin I and Rab3a in total brain homogenates from treated rats after E-2 administration. In striking contrast, the levels of all of these exocytotic proteins, including cellubrevin, were notably decreased in pituitary glands of E-2-treated rats. In addition, no differences were observed in the in vitro basal and 8-Br-cAMP-induced prolactin (PRL) release between pituitary cells from control and E-2-treated rats, whereas TRH-induced PRL release in anterior pituitary cells from E-2-treated animals was higher than in control donors. In conclusion, this study shows that protein components of the exocytotic machinery are specifically down-regulated in the pituitary gland of E-2-treated Fischer 344 rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 06:46:42