Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A fluorescence-based high throughput screen for the transporter associatedwith antigen processing
Autore:
Blevitt, JM; Fruh, K; Glass, C; Jackson, MR; Peterson, PA; Huang, SM;
Indirizzi:
RW Johnson Pharmaceut Res Inst, San Diego, CA 92121 USA RW Johnson Pharmaceut Res Inst San Diego CA USA 92121 Diego, CA 92121 USA
Titolo Testata:
JOURNAL OF BIOMOLECULAR SCREENING
fascicolo: 2, volume: 4, anno: 1999,
pagine: 87 - 91
SICI:
1087-0571(199904)4:2<87:AFHTSF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLASS-I MOLECULE; PEPTIDE TRANSPORTER; CELL BIOLOGY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Huang, SM RWAJohnson Pharmaceut Res Inst, 3535 Gen Atom Court,Suite 100, San Diego, C RW Johnson Pharmaceut Res Inst 3535 Gen Atom Court,Suite 100 San Diego CA USA 92121
Citazione:
J.M. Blevitt et al., "A fluorescence-based high throughput screen for the transporter associatedwith antigen processing", J BIOMOL SC, 4(2), 1999, pp. 87-91

Abstract

The transporter associated with antigen processing (TAP) is essential for antigen presentation by major histocompatibility complex (MHC) class I molecules. Traditional methods used to analyze peptide transport mediated by TAP require radioactive labeling of peptides and time-consuming manipulation of Concanavalin A-Sepharose, Drug discovery research requires rapid and reliable evaluation of large number of samples for bioactivity, To meet these requirements a nonradioactive, HTS assay for peptide transport activity of TAP has been developed, The radioactive label in the traditional assays hasbeen replaced by a fluorescent label without compromising the transport efficiency of labeled peptide or the sensitivity of the assay. The use of multiscreen filtration plates has facilitated higher throughput and eliminatedthe centrifugation steps used in traditional TAP assays. The HTS assay shows similar kinetic characteristics as compared to the traditional assay. The HTS assay has been adapted on a Quadra(TM) 96-320 96-channel pipetting station (Tomtec, Hamden, CT) by optimizing time course, dose response of TAP to peptides and adenosine triphosphate (ATP), signal/noise ratio, reproducibility, and reagent stability. This HTS system has been utilized to screen a multiplexed compound library with a maximum of throughput 17,600 compounds per week.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:55:26