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Titolo:
HIV-1 Rev promotes the nuclear export of unspliced and singly spliced RNAsin a mammalian cell-free export system
Autore:
Huffman, KM; Arrigo, SJ; Schmidt, MG;
Indirizzi:
Med Univ S Carolina, Dept Microbiol & Immunol, Charleston, SC 29425 USA Med Univ S Carolina Charleston SC USA 29425 nol, Charleston, SC 29425 USA
Titolo Testata:
JOURNAL OF BIOMEDICAL SCIENCE
fascicolo: 3, volume: 6, anno: 1999,
pagine: 194 - 205
SICI:
1021-7770(199905/06)6:3<194:HRPTNE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATE REPLACEMENT VECTOR; MESSENGER-RNA; ACTIVATION DOMAIN; PLASMA-MEMBRANES; RAT-LIVER; TRANSPORT; PROTEIN; EXPRESSION; SIGNAL; IDENTIFICATION;
Keywords:
HIV-1 Rev; nuclear export; cis acting repressor sequences; HIV-1 protease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt, MG MedAshleyS Carolina, Dept Microbiol & Immunol, Basic Sci Bldg,Room 201,171 Med Univ S Carolina Basic Sci Bldg,Room 201,171 Ashley Ave Charleston SC USA 29425
Citazione:
K.M. Huffman et al., "HIV-1 Rev promotes the nuclear export of unspliced and singly spliced RNAsin a mammalian cell-free export system", J BIOMED SC, 6(3), 1999, pp. 194-205

Abstract

Rev has been shown to promote the export of HIV-1 RNAs from Xenopus oocytenuclei, but a system to examine the direct effect of Rev on HIV-1 RNA export in mammalian somatic cells does not exist. In this report, the development of a cell-free RNA export system using COS cells is described. This system is capable of examining the movement of RNA from nuclei of COS cells transfected with an HIV-1 proviral construct into reconstituted cytosol from nontransfected cells. A reproducible preparation of nuclei free of residual cytoplasmic RNA is demonstrated. Export of RNA from these nuclei into reconstituted cell-free extracts was saturable and dependent on temperature and energy. Further validation of the system was obtained by confirming that the nuclear export of HIV-1-unspliced and partially spliced RNAs was dependent upon the expression of HIV-1 Rev and that the presence of Rev appeared todecrease the export of an HIV-1-spliced RNA. The system was also able to demonstrate that Rev did not appear to significantly Enhance the export of an HIV-1 protease-containing RNA that has been shown to be dependent upon Rev for maximal expression. Consequently, the system appears useful for the examination of parameters of nuclear export of HIV-1 and cellular RNAs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:07:12