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Titolo:
Increased intracellular cAMP renders HL-60 cells resistant to cytotoxicityof taxol
Autore:
Pae, HO; Yoo, JC; Jun, CD; Paik, SG; Choi, BM; Baek, KH; Kim, JM; Chung, HT;
Indirizzi:
Wonkwang Univ, Sch Med, Dept Microbiol & Immunol, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 mmunol, Iksan 570749, South Korea Wonkwang Univ, Med Resources Res Ctr, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 es Ctr, Iksan 570749, South Korea Chungnam Natl Univ, Coll Nat Sci, Dept Biol, Taejon, South Korea Chungnam Natl Univ Taejon South Korea i, Dept Biol, Taejon, South Korea Wonkwang Univ, Sch Med, Dept Neurosurg, Iksan 570749, South Korea WonkwangUniv Iksan South Korea 570749 rosurg, Iksan 570749, South Korea
Titolo Testata:
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
fascicolo: 2, volume: 21, anno: 1999,
pagine: 233 - 245
SICI:
0892-3973(1999)21:2<233:IICRHC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; DEPENDENT PROTEIN-KINASE; BREAST-CANCER; PHASE-II; EXPRESSION; MICROTUBULE; PACLITAXEL; MECHANISMS; CISPLATIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, HT Wonkwang Univ, Sch Med, Dept Microbiol & Immunol, Iksan 570749, South Korea Wonkwang Univ Iksan South Korea 570749 san 570749, South Korea
Citazione:
H.O. Pae et al., "Increased intracellular cAMP renders HL-60 cells resistant to cytotoxicityof taxol", IMMUNOPH IM, 21(2), 1999, pp. 233-245

Abstract

The treatment of advanced cancers with paclitaxel (taxol) is hindered by the development of drug resistance. Resistance to taxol is known to be associated with multidrug resistance (MDR) and a mutation affecting either the alpha- or beta-subunit of tubulin. In this study, we demonstrated that an intracellular cAMP level may also play an important role in resistance to taxol in HL-60, acute promyelocytic leukemia cells. Exposure of HL-60 cells tovarious doses of taxol for 18 hr resulted in cell death. However, pretreatment of the cells with cAMP analogs such as N-6 : O-2-dibutyl CAMP (Db-cAMP), 8-(4-chlorophenylthio) cAMP (CPT-cAMP) and 8-bromo-cAMP (8-Br-cAMP) or an intracellular cAMP elevating agent such as forskolin apparently rendered HL-60 cells more resistant to taxol, but not with dimethyl sulfoxide (DMSO)or retinoic acid (RA), well known differentiating agents. To investigate whether protein kinase A (PKA) activated by an increase in intracellular cAMP level could be involved in increased taxol resistance of the cells, we examined the effects of PKA inhibitors, including H-89 and KT5720, on taxol resistance induced by Db-cAMP. The PKA inhibitors significantly abolished Db-cAMP-induced taxol resistance. These results suggest that cAMP analogs mayrender tumor cells more resistant to taxol via PKA activation.

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Documento generato il 30/11/20 alle ore 02:28:26