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Titolo:
Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells
Autore:
Ishimura, A; Princler, GL; Lin, JJ; Kung, HF; Maeno, M;
Indirizzi:
Niigata Univ, Fac Sci, Dept Biol, Niigata 9502181, Japan Niigata Univ Niigata Japan 9502181 ci, Dept Biol, Niigata 9502181, Japan NCI,erick,rick Canc Res & Dev Ctr, Div Basic Sci, Lab Biochem Physiol, Fred NCI Frederick MD USA 21702 Ctr, Div Basic Sci, Lab Biochem Physiol, Fred
Titolo Testata:
GROWTH FACTORS
fascicolo: 3, volume: 16, anno: 1999,
pagine: 171 - 177
SICI:
0897-7194(1999)16:3<171:IOXBMP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-PROTEINASE; DROSOPHILA; GENE; EXPRESSION; HOMOLOG;
Keywords:
tolloid; dorso-ventral axis; BMP-1; processing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Maeno, M Niigata Univ, Fac Sci, Dept Biol, Niigata 9502181, Japan Niigata Univ Niigata Japan 9502181 Biol, Niigata 9502181, Japan
Citazione:
A. Ishimura et al., "Immunodetection of Xenopus bone morphogenetic protein-1 in adult and embryonic cells", GROW FACTOR, 16(3), 1999, pp. 171-177

Abstract

In order to analyze biochemical properties of Xenopus bone morphogenetic protein-1 (XBMP-1), rabbit antiserum (alpha-B1) was raised against a synthetic peptide (P1) corresponding to a hydrophilic N-terminal region. XBMP-1B (Xtld) synthesized in the reticulocyte lysate was successfully immunoprecipitated by this antiserum. This precipitation was completely blocked when Pi was added to the reaction, indicating that alpha-B1 recognized XBMP-1B specifically. In Western blot analysis, two distinct sizes of protein (107 and 34 kD) were detected in hind limbs in metamorphosing animals. Both proteinswere detected in various adult tissues such as lung, liver, kidney, heart,muscle, intestine, brain, and testis, The mixing of the liver and muscle extracts, and the following detection of immunoreactive proteins suggested that the 34 kD band was a proteolytic product of the 107 kD protein. In the embryonic extracts from the unfertilized egg (stage 0) to swimming tadpoles(stage 40), a 63 kD protein was detected in addition to the 107 kD protein. We also showed that the 107 kD protein was much more expressed in the animal half of the unfertilized eggs than in the vegetal half, but that it wasubiquitously expressed in the gastrula embryos. We suggest that the 63 and107 kD proteins correspond to full-length proteins encoded by XBMP-1A and XBMP-1B genes, and these proteins are expressed in embryo and in various adult tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:26:03