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Titolo:
Cloning and characterization of mdc genes encoding malonate decarboxylase from Pseudomonas putida
Autore:
Chohnan, S; Kurusu, Y; Nishihara, H; Takamura, Y;
Indirizzi:
Ibaraki Univ, Sch Agr, Dept Bioresource Sci, Ibaraki, Osaka 3000393, JapanIbaraki Univ Ibaraki Osaka Japan 3000393 i, Ibaraki, Osaka 3000393, Japan
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 174, anno: 1999,
pagine: 311 - 319
SICI:
0378-1097(19990515)174:2<311:CACOMG>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACYL-CARRIER PROTEIN; MALONOMONAS-RUBRA; ESCHERICHIA-COLI; ACINETOBACTER-CALCOACETICUS; NUCLEOTIDE-SEQUENCE; PROSTHETIC GROUP; FABD GENE; ENZYME; EXPRESSION; CLUSTER;
Keywords:
malonate decarboxylase; Pseudomonas putida; DNA sequence; expression in Escherichia coli;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Takamura, Y Ibaraki3000393,ch Agr, Dept Bioresource Sci, 3-21-1 Chu Ou, Ibaraki, Osaka Ibaraki Univ 3-21-1 Chu Ou Ibaraki Osaka Japan 3000393 , Osaka
Citazione:
S. Chohnan et al., "Cloning and characterization of mdc genes encoding malonate decarboxylase from Pseudomonas putida", FEMS MICROB, 174(2), 1999, pp. 311-319

Abstract

The DNA fragment encoding malonate decarboxylase, involved in malonate assimilation, was cloned from Pseudomonas putida. The Il-kb DNA fragment contained nine open reading frames, which were designated mdcABCDEGHLM in the given order. N-terminal protein sequencing established that the mdcA, mdcC, mdcD, mdcE and mdcH genes encoded subunits alpha, delta, beta, gamma and epsilon Of the malonate decarboxylase, respectively. Malonate decarboxylase was functionally expressed in Escherichia coli from plasmid harboring the entire gene cluster or the mdc genes lacking the mdcL and mdcM genes. The mdcLand mdcM genes encode membrane proteins and disruption of the genes of P. putida by the insertion of a kanamycin resistance cassette reduced the malonate uptake activity of the organism. Thus, we conclude that MdcLM is a malonate transporter. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 15:12:46