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Titolo:
Sustained proliferation of PDX-1(+) cells derived from human islets
Autore:
Beattie, GM; Itkin-Ansari, P; Cirulli, V; Leibowitz, G; Lopez, AD; Bossie, S; Mally, MI; Levine, F; Hayek, A;
Indirizzi:
Univ Calif San Diego, Ctr Mol Genet, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 enet, San Diego, CA 92103 USA Univ Calif San Diego, Whittier Inst, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 Inst, San Diego, CA 92103 USA Univ Calif San Diego, Dept Pediat, San Diego, CA 92103 USA Univ Calif San Diego San Diego CA USA 92103 diat, San Diego, CA 92103 USA
Titolo Testata:
DIABETES
fascicolo: 5, volume: 48, anno: 1999,
pagine: 1013 - 1019
SICI:
0012-1797(199905)48:5<1013:SPOPCD>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC ENDOCRINE-CELLS; HEPATOCYTE GROWTH-FACTOR; HUMAN FETAL PANCREAS; ADHESION MOLECULE; NEONATAL RAT; EXTRACELLULAR-MATRIX; BETA-CELLS; EP-CAM; CULTURE; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Hayek, A Univa,alif San Diego, Dept Pediat, Islet Res Lab, 9894 Genesee Ave, La Joll Univ Calif San Diego 9894 Genesee Ave La Jolla CA USA 92037 Joll
Citazione:
G.M. Beattie et al., "Sustained proliferation of PDX-1(+) cells derived from human islets", DIABETES, 48(5), 1999, pp. 1013-1019

Abstract

Ex vivo expansion of human beta-cells is an important step toward the development of cell-based insulin delivery systems in type 1 diabetes. Here, wereport that human pancreatic endocrine cells can be expanded through 15 cell doublings in vitro for an estimated total 30,000-fold increase in cell number. mie believe that the cells resulting from these cultures are of beta-cell origin, since they uniformly express the transcription factor PDX-1 (STF-1, IDX-1, IPF-1), which is initially seen only in cells positive for insulin and negative for the ductal cell marker cytokeratin (CK)-19. To rule out the possibility that PDX-1 expression might be induced by the culture conditions used here, cells from isolated human pancreatic ducts were cultured under the same conditions as the islet cells. Cells in these cultures expressed CK-19 but not PDX-1. Although the expanded beta-cells continued to express PDX-1, insulin expression was lost over time. Whether reexpression of islet-specific genes in vitro is essential for successful cell transplantation remains to be determined.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:23:49