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Titolo:
Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro
Autore:
Reischl, J; Prelle, K; Schol, H; Neumuller, C; Einspanier, R; Sinowatz, F; Wolf, E;
Indirizzi:
Univ Munich, Dept Mol Anim Breeding & Genet, D-85764 Munich, Germany Univ Munich Munich Germany D-85764 ding & Genet, D-85764 Munich, Germany Tech Univ Munchen Weihenstephan, Inst Physiol, D-8050 Freising, Germany Tech Univ Munchen Weihenstephan Freising Germany D-8050 reising, Germany Univ Munich, Inst Comparat Trop Med & Parasitol, Munich, Germany Univ Munich Munich Germany mparat Trop Med & Parasitol, Munich, Germany Univ Munich, Inst Vet Anat 2, Munich, Germany Univ Munich Munich Germany niv Munich, Inst Vet Anat 2, Munich, Germany
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 2, volume: 296, anno: 1999,
pagine: 371 - 383
SICI:
0302-766X(199905)296:2<371:FAPADO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTRUS-ASSOCIATED PROTEIN; IN-VITRO; IMMUNOCYTOCHEMICAL LOCALIZATION; CONDITIONED MEDIUM; BLASTOCYST STAGE; RABBIT OVIDUCTS; ZONA-PELLUCIDA; ESTROUS-CYCLE; CULTURE; INVITRO;
Keywords:
oviduct epithelium; perfusion culture; cell support; permeable membranes; reverse transcription-polymerase chain reaction bovine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Wolf, E UnivGermany, Dept Mol Anim Breeding & Genet, Hackerstr 27, D-85764Munich, Univ Munich Hackerstr 27 Munich Germany D-85764 , D-85764 Munich,
Citazione:
J. Reischl et al., "Factors affecting proliferation and dedifferentiation of primary bovine oviduct epithelial cells in vitro", CELL TIS RE, 296(2), 1999, pp. 371-383

Abstract

The oviduct is the physiological site for key events in reproduction, suchas capacitation of spermatozoa, fertilization and early embryonic development. Interactions between oviduct epithelial cells and gametes or embryos cannot sufficiently be studied in vivo. Therefore, model systems are needed which mimic in vivo conditions most closely. In this study we optimised themethod for isolating bovine oviduct cells and compared different cell support materials as well as two culture systems (perfusion vs static culture) for their ability to maintain characteristic morphological and functional features of oviduct cells. Out of nine different cell support materials tested, cellulose nitrate (0.45 mu m pore size) was the most suitable to maintain cells in a manner similar to freshly isolated oviduct epithelial cells. Comparing static vs perfusion culture by electron microscopy, morphologicaldifferences of the cells were insignificant in the first days of culture, while they became more evident after 8 days. The cells in the static systemlost typical characteristics such as columnar shape, cilia and secretory protrusions, while these features were still present in perfusion culture. In addition, intense ciliogenesis and cytoplasmic organelles for protein synthesis were found under perfusion conditions. These findings were underlined by differences in expression of the oviduct-specific oestrus-associated glycoprotein 85-97 kDa (GP 85-97) gene as revealed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The RNA levels of this specific gene were significantly higher in perfusion compared to the static culture system. Our data show clear advantages of perfusion vs static culture for primary bovine oviduct epithelial cells.

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Documento generato il 26/11/20 alle ore 20:05:14