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Titolo:
Prilocaine induces apoptosis in osteoblastic cells
Autore:
Nakamura, K; Kido, H; Morimoto, Y; Morimoto, H; Kobayashi, S; Morikawa, M; Haneji, T;
Indirizzi:
Kyushu580,t Coll, Dept Oral Anat 1, Kokurakita Ku, Kitakyushu, Fukuoka 8038 Kyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 itakyushu, Fukuoka 8038 Kyushu8038580,ll, Dept Prosthet Dent 1, Kokurakita Ku, Kitakyushu, FukuokaKyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 Ku, Kitakyushu, Fukuoka Kyushu580,t Coll, Dept Dent Radiol, Kokurakita Ku, Kitakyushu, Fukuoka 8038 Kyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 itakyushu, Fukuoka 8038
Titolo Testata:
CANADIAN JOURNAL OF ANAESTHESIA-JOURNAL CANADIEN D ANESTHESIE
fascicolo: 5, volume: 46, anno: 1999,
parte:, 1
pagine: 476 - 482
SICI:
0832-610X(199905)46:5<476:PIAIOC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEWBORN MOUSE CALVARIA; PROTEIN PHOSPHATASE INHIBITORS; LOCAL-ANESTHETIC INHIBITION; OKADAIC ACID; CALYCULIN-A; LINE; 1-ALPHA,25-DIHYDROXYVITAMIN-D3; MORPHOLOGY; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Haneji, T Kyushuhu,nt Coll, Dept Oral Anat 1, Kokurakita Ku, 2-6-1 Manazuru, Kitakyus Kyushu Dent Coll 2-6-1 Manazuru Kitakyushu Fukuoka Japan 8038580
Citazione:
K. Nakamura et al., "Prilocaine induces apoptosis in osteoblastic cells", CAN J ANAES, 46(5), 1999, pp. 476-482

Abstract

Purpose: To determine whether prilocaine, a local anesthetic, induces apoptosis in osteoblastic cells. Methods: After reaching subconfluence, human osteoblastic Saos-2 and MG63 cells and mouse osteoblastic MC3T3-EI cells were exposed for 48 hr to varying concentration; of prilocaine up to 10 mM and the cytotoxicity of the cells was analyzed by phase-contrast microscopy and WST-I assay. Saos-2 cells treated for 48 hr with 5 mM prilocaine were stained with Hoechst 33342 and nuclear fragmentation was examined under a fluorescence microscope. DNA wasextracted from the cells treated with 5 mM prilocaine and DNA ladder formation (a hallmark of apoptosis) was analyzed by agarose gel electrophoresis. Result: Prilocaine induced-cell death in Saos-2 cells in a dose- and time-dependent manner up to the concentration of 10 mM. Marked nuclear condensation and fragmentation of chromatin were observed in the prilocaine treated cells. DNA ladder formation also was induced by prilocaine treatment. Prilocaine induced DNA ladder formation was dose;dependent with maximal effect at a concentration of 5 mM and was time-dependent from 12 to 48 hr. DNA ladder formation was also induced by prilocaine treatment in human osteoblasticMG63 cells and mouse osteoblastic MC3T3-EI cells. Cycloheximide prevented prilocaine-induced apoptosis in Saos-2 cells in a dose-dependent fashion upto 20 mu M as determined by WST-I assay and DNA ladder formation in agarose gel electrophoresis. Conclusion: Osteoblastic cells treated with prilocaine exhibit both morphological and biochemical features indicative of apoptosis. The apoptotic mechanisms involve transcriptional regulation of specific proteins or protein synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:25:31