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Titolo:
Neuropeptide Y (NPY) expression is increased in explanted guinea pig parasympathetic cardiac ganglia neurons
Autore:
Lynch, SW; Braas, KM; Harakall, SA; Kennedy, AL; Mawe, GM; Parsons, RL;
Indirizzi:
Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Neurobiol, Burlington, VT 05405 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 827, anno: 1999,
pagine: 70 - 78
SICI:
0006-8993(19990508)827:1-2<70:NY(EII>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASOACTIVE-INTESTINAL-PEPTIDE; SUPERIOR CERVICAL-GANGLION; INTRACARDIAC NEURONS; SYMPATHETIC-GANGLIA; PERIPHERAL AXOTOMY; INTRINSIC NEURONS; MESSENGER-RNA; SPINAL-CORD; HEART; RAT;
Keywords:
cardiac ganglia; parasympathetic neuron; immunocytochemistry; reverse transcription polymerase chain reaction; neuropeptide Y; microtubule-associated protein-2; colchicine; NPY mRNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Parsons, RL Univ Vermont, Coll Med, Dept Anat & Neurobiol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Burlington, VT 05405 USA
Citazione:
S.W. Lynch et al., "Neuropeptide Y (NPY) expression is increased in explanted guinea pig parasympathetic cardiac ganglia neurons", BRAIN RES, 827(1-2), 1999, pp. 70-78

Abstract

While expression of neuropeptides by sympathetic neurons is altered by decentralization and axotomy, it is not known whether similar experimental paradigms also modulate the chemical phenotype of parasympathetic cardiac ganglia neurons. The present study tested whether guinea pig parasympathetic neuron neuropeptide Y (NPY) expression was altered when cardiac ganglia preparations were maintained as organ explants in the presence or absence of colchicine. Two experimental approaches were used to examine NPY expression. First, immunocytochemical techniques were used to quantitate numbers of neurons within the cardiac ganglia exhibiting NPY-immunoreactivity; second, reverse transcription PCR was used to examine proNPY mRNA expression. In control cardiac ganglia preparations, approximately 4% of ganglia neurons exhibited NPY-immunoreactivity. The percentage of NPY-immunopositive neurons in 30- and 72-h explanted cardiac ganglia preparations, maintained in the absence of colchicine, increased to 11 and 16%, respectively. Colchicine treatment of explanted preparations further increased the percentage of NPY-positive ganglia cells 24% (30 h) and 32% (72 h). All NPY-immunoreactive neurons from control ganglia and explanted ganglia were choline acetyltransferase(ChAT)-immunoreactive, indicating retention of the cholinergic phenotype. ProNPY mRNA also was increased following ganglia explantation, consistent withthe increase in the numbers of NPY-immunoreactive neurons. NPY transcriptswere further increased after 30 h, but not after 72 h in colchicine-treated, explanted cardiac ganglia preparations. These results demonstrate that NPY expression is altered in explanted cardiac ganglia preparations, providing evidence that the chemical phenotype of parasympothetic cardiac neurons can be modulated. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:07:37