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Titolo:
alpha-MSH and its receptors in regulation of tumor necrosis factor-alpha production by human monocyte macrophages
Autore:
Taherzadeh, S; Sharma, S; Chhajlani, V; Gantz, I; Rajora, N; Demitri, MT; Kelly, L; Zhao, H; Ichiyama, T; Catania, A; Lipton, JM;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Physiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 ed Ctr, Dept Physiol, Dallas, TX 75235 USA Univ5Texas, SW Med Ctr, Dept Anesthesiol & Pain Management, Dallas, TX 7523 Univ Texas Dallas TX USA 75235 thesiol & Pain Management, Dallas, TX 7523 Astra Hassle AB, S-43183 Molndal, Sweden Astra Hassle AB Molndal Sweden S-43183 assle AB, S-43183 Molndal, Sweden Univ Michigan, Med Ctr, Dept Surg, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 , Dept Surg, Ann Arbor, MI 48109 USA IRCCS Osped Maggiore, Div Internal Med 3, I-20122 Milan, Italy IRCCS OspedMaggiore Milan Italy I-20122 nal Med 3, I-20122 Milan, Italy
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
fascicolo: 5, volume: 45, anno: 1999,
pagine: R1289 - R1294
SICI:
0363-6119(199905)45:5<R1289:AAIRIR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING HORMONE; MOLECULAR-CLONING; MELANOCORTIN RECEPTOR; NITRIC-OXIDE; MODULATION; CELLS; INFLAMMATION; EXPRESSION; BRAIN;
Keywords:
melanocortin peptides; melanocortin receptors; inflammation; autocrine regulation; neuroimmunomodulation; THP-1 cells; melanocortin-1 receptor antibody; alpha-melanocyte-stimulating hormone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Lipton, JM Univ35exas, SW Med Ctr, Dept Physiol, 5323 Harry Hines Blvd, Dallas, TX 752 Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 as, TX 752
Citazione:
S. Taherzadeh et al., "alpha-MSH and its receptors in regulation of tumor necrosis factor-alpha production by human monocyte macrophages", AM J P-REG, 45(5), 1999, pp. R1289-R1294

Abstract

The hypothesis that macrophages contain an autocrine circuit based on melanocortin [ACTH and alpha-melanocyte-stimulating hormone (alpha-MSH)] peptides has major implications for neuroimmunomodulation research and inflammation therapy. To test this hypothesis, cells of the THP-1 human monocyte/macrophage line were stimulated with lipopolysaccharide (LPS) in the presence and absence of alpha-MSH. The inflammatory cytokine tumor necrosis factor (TNF)-alpha was inhibited in relation to alpha-MSH concentration. Similar inhibitory effects on TNF-alpha were observed with ACTH peptides that contain the ol-MSH amino acid sequence and act on melanocortin receptors. Nuclease protection assays indicated that expression of the human melanocortin-1 receptor subtype (hMC-1R) occurs in THP-1 cells; Southern blots of RT-PCR product revealed that additional subtypes, hMC-3R and hMC-5R, also occur. Incubation of resting macrophages with antibody to hMC-1R increased TNF-alpha concentration; the antibody also markedly reduced the inhibitory influence ofalpha-MSH on TNF-alpha in macrophages treated with LPS. These results in cells known to produce alpha-MSH at rest and to increase secretion of the peptide when challenged are consistent with an endogenous regulatory circuit based on melanocortin peptides and their receptors. Targeting of this neuroimmunomodulatory circuit in inflammatory diseases in which myelomonocytic cells are prominent should be beneficial.

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Documento generato il 29/09/20 alle ore 22:34:39