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Titolo:
Mitogen-induced proliferation increases biotin uptake into human peripheral blood mononuclear cells
Autore:
Zempleni, J; Mock, DM;
Indirizzi:
Arkansas Childrens Hosp, Res Inst, Little Rock, AR 72202 USA Arkansas Childrens Hosp Little Rock AR USA 72202 ittle Rock, AR 72202 USA UniveArkansas Med Sci, Dept Pediat, Div Gastroenterol Hepatol & Nutr, Littl Univ Arkansas Med Sci Little Rock AR USA 72202 erol Hepatol & Nutr, Littl
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 45, anno: 1999,
pagine: C1079 - C1084
SICI:
0363-6143(199905)45:5<C1079:MPIBUI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FUNCTIONAL EXPRESSION; HUMAN PLASMA; LIPOIC ACID; HUMAN SERUM; LYMPHOCYTES; TRANSPORTER; DEFICIENCY; HISTONES; CLONING;
Keywords:
concanavalin A; lipoic acid; phytohemagglutinin; pokeweed; transport kinetics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Mock, DM Arkansas2202ldrens Hosp, Dept Pediat GI, 800 Marshall St, Little Rock, AR 7 Arkansas Childrens Hosp 800 Marshall St Little Rock AR USA 72202
Citazione:
J. Zempleni e D.M. Mock, "Mitogen-induced proliferation increases biotin uptake into human peripheral blood mononuclear cells", AM J P-CELL, 45(5), 1999, pp. C1079-C1084

Abstract

We sought to determine whether the proliferation of immune cells affects the cellular uptake of the vitamin biotin. Peripheral blood mononuclear cells (PBMC) were isolated from healthy adults. The proliferation of PBMC was induced by either pokeweed lectin, concanavalin A, or phytohemagglutinin. When the medium contained a physiological concentration of [H-3]biotin, nonproliferating PBMC accumulated 406 +/- 201 amol [H-3]biotin.10(6) cells(-1).30 min(-1). For proliferating PBMC, [H-3]biotin uptake increased to between 330 and 722% of nonproliferating values. Maximal transport rates of [H-3]biotin in proliferating PBMC were also about four times greater than those innonproliferating PBMC, suggesting that proliferation was associated with an increase in the number of biotin transporters on the PBMC membrane. The biotin affinities and specificities of the transporter for proliferating andnonproliferating PBMC were similar, providing evidence that the same transporter mediates biotin uptake in both states. [C-14]urea uptake values for proliferating and nonproliferating PBMC were similar, suggesting that the increased [H-3]biotin uptake was not caused by a global upregulation of transporters during proliferation. We conclude that PBMC proliferation increases the cellular accumulation of biotin.

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Documento generato il 17/01/21 alle ore 18:10:57