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Titolo:
Metabolic adaptation of endothelial cells to substrate deprivation
Autore:
Culic, O; Decking, UKM; Schrader, J;
Indirizzi:
Univ Dusseldorf, Inst Herz & Kreislaufphysiol, D-40225 Dusseldorf, GermanyUniv Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 5, volume: 45, anno: 1999,
pagine: C1061 - C1068
SICI:
0363-6143(199905)45:5<C1061:MAOECT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATP TURNOVER RATES; PROTEIN-SYNTHESIS; ADULT-RAT; HYPOXIA; HEPATOCYTES; INHIBITORS; MYOCARDIUM; RECOVERY; HEART;
Keywords:
nucleotide; purine de novo synthesis; protein synthesis; energy deprivation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Schrader, J Univ Dusseldorf, Dept Physiol, POB 10 10 07, D-40001 Dusseldorf, Germany Univ Dusseldorf POB 10 10 07 Dusseldorf Germany D-40001 rmany
Citazione:
O. Culic et al., "Metabolic adaptation of endothelial cells to substrate deprivation", AM J P-CELL, 45(5), 1999, pp. C1061-C1068

Abstract

Endothelial cells are known to be metabolically rather robust. To study the mechanisms involved, porcine aortic endothelial cells (PAEC), cultured onmicrocarrier beads, were perfused with glucose (10 mM) or with substrate-free medium. Substrate-free pel fusion for 2 h induced an almost complete loss of nucleoside triphosphates (P-31-NMR) and decreased heat flux, a measure of total energy turnover, by >90% in parallel microcalorimetric measurements. Heat flux and nucleoside triphosphates recovered after addition of glucose. Because protein synthesis is a major energy consumer in PAEC, the rate of protein synthesis was measured ([C-14]leucine incorporation). Reduction or blockade of energy supply resulted in a pronounced reduction in the rate of protein synthesis (up to 80% reduction). Intracellular triglyceride stores were decreased by similar to 60% after 2 h of substrate-free perfusion. Under basal perfusion conditions, PAEC released similar to 30 pmol purine mg protein(-1).min(-1), i.e., 16% of the cellular ATP per hour, while ATPremained constant. Substrate deprivation increased the release of various purines and pyrimidines about threefold and also induced a twofold rise in purine de novo synthesis ([14C]formate). These results demonstrate that PAEC are capable of recovering from extended periods of substrate deprivation. They can do so by a massive downregulation of their energy expenditure, particularly protein synthesis, while at the same time using endogenous triglycerides as substrates and upregulating purine de novo synthesis to compensate for the loss of purines.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 07:14:00