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Titolo:
Chemistry of photoproteins as interface between bioactive molecules and protein function
Autore:
Isobe, M; Fujii, T; Suwan, S; Kuse, M; Tsuboi, K; Miyazaki, A; Feng, MC; Li, JM;
Indirizzi:
Nagoya Univ, Sch Bioagr Sci, Organ Chem Lab, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 Lab, Nagoya, Aichi 4648601, Japan
Titolo Testata:
PURE AND APPLIED CHEMISTRY
fascicolo: 11, volume: 70, anno: 1998,
pagine: 2085 - 2092
SICI:
0033-4545(199811)70:11<2085:COPAIB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIREFLY BIOLUMINESCENCE SYSTEM; OKADAIC ACID; LOW-TEMPERATURE; LUMINOUS SQUID; TAUTOMYCIN; PHOTOOXYGENATION; (+)-TAUTOMYCIN; DIOXETANONE; INHIBITORS; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Isobe, M Nagoya Univ, Sch Bioagr Sci, Organ Chem Lab, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 oya, Aichi 4648601, Japan
Citazione:
M. Isobe et al., "Chemistry of photoproteins as interface between bioactive molecules and protein function", PUR A CHEM, 70(11), 1998, pp. 2085-2092

Abstract

Bioluminescence of Okinawan squid includes a photoprotein named "Symplectin" which gives light by addition of Na+ (or K+). The chromophore is covalently bound-imidazopyrazinone analog to protein. Symplectin was extracted with 0.6M KCl and was analyzed to be composed of 3 different molecular-size proteins. Symplectin A, the smallest photoproteins, was purified by gel permeation chromatography to have 15kd by MALDI/TOF mass spectrometry. The molecular mechanism of this luminescence is described. Bioluminescence is also applied for trace analyses and the chromophore in some new fireflies is chemically analyzed. We made a very sensitive detection system of luciferin to a detection limit of 10(-16) mol/10 mu l by using immobilized luciferase and a single photon-counter. An example of the firefly luminescence application is demonstrated in an inhibitory-assay of protein phosphatase activity by inhibitors in the same class of tautomycin/okadaic acid.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:30:39