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Titolo:
Expression of human p53 requires synergistic activation of transcription from the p53 promoter by AP-1, NF-kappa B and Myc/Max
Autore:
Kirch, HC; Flaswinkel, S; Rumpf, H; Brockmann, D; Esche, H;
Indirizzi:
Univanysen Gesamthsch, Sch Med, Inst Mol Biol Canc Res, D-45122 Essen, Germ Univ Essen Gesamthsch Essen Germany D-45122 anc Res, D-45122 Essen, Germ
Titolo Testata:
ONCOGENE
fascicolo: 17, volume: 18, anno: 1999,
pagine: 2728 - 2738
SICI:
0950-9232(19990429)18:17<2728:EOHPRS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOCELLULAR-CARCINOMA; INDUCIBLE ENHANCER ELEMENTS; CYCLE CHECKPOINT PATHWAY; TUMOR-SUPPRESSOR GENE; NECROSIS-FACTOR-ALPHA; HELIX-LOOP-HELIX; WILD-TYPE P53; BINDING PROTEIN; CANCER-CELLS; DNA-BINDING;
Keywords:
p53; promoter; transcription; AP-1; NF-kappa B; Myc/Max;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Kirch, HC Univ5122en Gesamthsch, Sch Med, Inst Mol Biol Canc Res, Hufelandstr 55, D-4 Univ Essen Gesamthsch Hufelandstr 55 Essen Germany D-45122 D-4
Citazione:
H.C. Kirch et al., "Expression of human p53 requires synergistic activation of transcription from the p53 promoter by AP-1, NF-kappa B and Myc/Max", ONCOGENE, 18(17), 1999, pp. 2728-2738

Abstract

Transcriptional control of p53 expression participates in the generation of appropriate le, els of active p53 in response to mitogenic stimulation, This prompted us to study the role of a putative AP-1 and a NF-kappa B motifin the human p53 promoter for transcriptional regulation, We show that mutation of the AP-1 or the NF-kappa B motif abolishes transcription from the human p53 promoter in HeLa, HepG2 and adenovirus type 5 E1-transformed 293 cells, In comparison, mutation of the previously characterized Myc/Max/USF binding site in the human p53 promoter reduces the transcription rate fivefold. The AP-1 motif in the human p53 promoter binds c-Fos and c-Jun and theNF-kappa-B motif binds p50(NF-kappa B1) and p65(RelA). The cooperative nature of transcriptional activation by these factors was documented by repression of c-fos or NF-kappa B1 translation: Pretreatment of the cells,vith a c-fos or p50(NF-kappa B1) antisense oligonucleotide suppresses transcription from the human p53 promoter completely. In addition, we show that (a) thelevel of endogenous p53 mRNA and (b) transcription from the strictly p53-dependent human mdm2 promoter are reduced in the presence of c-fos, c-jun, p50(NF-kappa B1), p65(RelA) or c-myc antisense oligonucleotides, underscoring the importance of these transcription factors for the expression of functional p53.

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Documento generato il 26/09/20 alle ore 11:53:20